n Methods Useful in Genetics and Oncology
As the T m-shift is mainly caused by the heteroduplices, mixing of samples with
wildtype-DNA is recommended to create heteroduplices in homozygote mutant
samples.
References
1. Ririe KM, Rasmussen RP, Wittwer CT (1997) Product differentiation by analysis of DNA
melting curves during the polymerase chain reaction. Anal Biochem 245:154-160
2. Lay MJ, Wittwer CT (1997) Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR. Clin Chern 43:2262-2267
3. Aoshima T, Sekido Y, Miyazaki T, Kajita M, Mimura S, Watanabe K, Shimokata K, Niwa T
(2000) Rapid detection of deletion mutations in inherited metabolic diseases by melting
curve analysis with LightCycier. Clin Chern 46:119-122
4. Zielenski J, Rozmahel R, Bozon D, Kerem B, Grzelczak Z, Riordan JR, Rommens J, Tsui LC
(1991) Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator
(CFTR) gene. Genomics 10:214-228
5. Wetmur JG (1995) in Molecular Biology and Biotechnology (Meyers, RA, Ed.), pp.605-608,
VCH, New York
6. Liechti-Gallati S, Schneider V, Neeser D, Kraemer R (1999) Two buffer PAGE system-based
SSCP/HD analysis: a general protocol for rapid and sensitive mutation screening in cystic
fibrosis and any other human genetic disease. Eur J Hum Genet 7:590-598
7. Bommarito S, Peyret N, Santa Lucia J Jr (2000) Nucl Acids Res 28:1929-1934
As the T m-shift is mainly caused by the heteroduplices, mixing of samples with
wildtype-DNA is recommended to create heteroduplices in homozygote mutant
samples.
References
1. Ririe KM, Rasmussen RP, Wittwer CT (1997) Product differentiation by analysis of DNA
melting curves during the polymerase chain reaction. Anal Biochem 245:154-160
2. Lay MJ, Wittwer CT (1997) Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR. Clin Chern 43:2262-2267
3. Aoshima T, Sekido Y, Miyazaki T, Kajita M, Mimura S, Watanabe K, Shimokata K, Niwa T
(2000) Rapid detection of deletion mutations in inherited metabolic diseases by melting
curve analysis with LightCycier. Clin Chern 46:119-122
4. Zielenski J, Rozmahel R, Bozon D, Kerem B, Grzelczak Z, Riordan JR, Rommens J, Tsui LC
(1991) Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator
(CFTR) gene. Genomics 10:214-228
5. Wetmur JG (1995) in Molecular Biology and Biotechnology (Meyers, RA, Ed.), pp.605-608,
VCH, New York
6. Liechti-Gallati S, Schneider V, Neeser D, Kraemer R (1999) Two buffer PAGE system-based
SSCP/HD analysis: a general protocol for rapid and sensitive mutation screening in cystic
fibrosis and any other human genetic disease. Eur J Hum Genet 7:590-598
7. Bommarito S, Peyret N, Santa Lucia J Jr (2000) Nucl Acids Res 28:1929-1934