n Methods Useful in Genetics and Oncology
A
B
0.690bp 36%GC
04 -
-0.2.() 4I;; 2;::..
~1.9~
i1.6~
0.80.6~
[4 -
I
68
I
ro
I
n
I
74
...........
I
76
..-:130bp 37%GC
}OObp 37%GC
1
E16
1
ee
200bp 42%GC
/
1
90
1
92
,200bp49%GC
I
I
I
I
79
80
82
84
r"""""".rcl
I
B6
I
8B
I
ill
I
92
.
!).t
Fig. lA, B. Melting curves of CFTR-fragments of different length and %Gc. A: product 1 (red),
product 2 (blue), product 3 (green); B: product 4 (blue), product 5 (red), product 6 (green), product 7 (yellow), H20-controls (pink, grey, anthracite, and black)
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