Genotyping by Guanosine-Dependent Quenching of Single-Labeled Fluorescein Probes . . ,
Table 1. Oligonucleotides
Hereditary hemochromatosis C282Y (GenBank Accession #Z92910)
Position
Length
GC(%) Tm (0C)
C282Y primers
TGGCAAGGGTAAACAGATCC
6443
20
50.0
62.5
TACCTCCTCAGGCACTCCTC
6838 R
20
60.0
64.9
Product
6443-6838 396
C282Y probe
F-CACCTGGCACGTATATCTCTG- P
6729R
21
52.4
62.8
Hereditary hemochromatosis H63D (GenBank Accession #Z92910)
H63D primers
CACATGGTTAAGGCCTGTTG
4620
20
50.0
62.2
GATCCCACCCTTTCAGACTC
4861 R
20
55.0
62.5
Product
4620-4861 242
H630 probe
P-CACACGGCGACTCTCATCATCATAGAAC- P
4779R
28
50.0
68.4
Cystic fibrosis F508del (GenBank Accession #M55115)
F508del primers
GGAGGCAAGTGAATCCTGAG
245
20
55.0
63.2
CCTCTTCTAGTTGGCATGCT
500 R
20
50.0
62.6
Product
245-500
256
F508del probe
F-ATAGGAAACACCAAAGATGATATTTTC-P
451 R
27
29.6
60.6
Methylenctctrahydrofolate reductase C677T (GenBank Accession #U09806)
C677T primers
TGAAGGAGAAGGTGTCTGCGGGA
653
23
56.5
68.7
AGGACGGTGCGGTGAGAGTG
See (13J
20
65.0
68.7
Product
198
C677T probe
F-TGCGTGATGATGAAATCGGCTCC-P
695 R
23
52.2
66.5
Factor V Lcidcn (G 1691 A; GcnBank Accession #L32764)
G1691A primers
TAATCTGTAAGAGCAGATCC
236
20
40.0
56.6
TGTTATCACACTGGTGCTAA
See (14)'
20
40.0
58.8
Product
187
G 1691A probe
F-CTGTATTCCTCGCCTGTC-P
276 R
18
55.6
59.9