Expression Analysis of Telomerase-Genes hTERT and hTR by Quantitative peR on LightCyder rpjJ
DNA and mRNA products were about the same, we chose to exclude these samples from further analysis.
In summary, we found that both the commercially available kits from Roche
Diagnostics and our own protocol give reproducible and accurate results. However, for better reproducibility and comparability between results obtained in different laboratories, we recommend using the standardized kits.
References
1. Shay JW, Bacchetti S (1997) A survey of telomerase activity in human cancer. Eur J Cancer
33:787-791
2. Healy KC (1995) Telomere dynamics and telomerase activation in tumor progression:
prospects for prognosis and therapy. Oncol Res 7:121-130
3. Poremba C, Bocker W, Willenbring H, Schafer KL, Otterbach F, Burger H et al. (1998) Telomerase activity in human proliferative breast lesions. Int J Oncol12:641-648
4. Poremba C, Shroyer KR, Frost M, Diallo R, Fogt F, Schafer K et al. (1999) Telomerase is a highly sensitive and specific molecular marker in fine-needle aspirates of breast lesions. J Clin
Oncol17:2020-2026
5. Poremba C, Willenbring H, Hero B, Christiansen H, Schafer KL, Brinkschmidt C et al. (1999)
Telomerase activity distinguishes between neuroblastomas with good and poor prognosis.
Ann OncollO:715-721
6. Morin GB (1989) The human telomere terminal transferase enzyme is a ribonucleoprotein
that synthesizes TTAGGG repeats. Cell 59:521-529
7. Ramakrishnan S, Eppenberger U, Mueller H, Shinkai Y, Narayanan R (1998) Expression profile of the putative catalytic subunit of the telomerase gene. Cancer Res 58:622-625
8. Avilion AA, Piatyszek MA, Gupta J, Shay JW, Bacchetti S, Greider CW (1996) Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. Cancer Res
56:645-650
9. Ito H, Kyo S, Kanaya T, Takakura M, Inoue M, Namiki M (1998) Expression of human telomerase subunits and correlation with telomerase activity in urothelial cancer. Clin Cancer Res
4: 1603-1608
10. Poremba C, Scheel C, Hero B, Christiansen H, Schaefer KL, Nakayama J et a1. (2000) Telomerase activity and telomerase subunits gene expression patterns in neuroblastoma: a molecular and immunohistochemical study establishing prognostic tools for fresh-frozen and
paraffin-embedded tissues. J Clin Oncol18:2582-2592
11. Poremba C, Hero B, Heine B, Scheel C, Schaefer KL, Christiansen H et al. (2000) Telomerase
is a strong indicator for assessing the proneness to progression in neuroblastomas. Med
Pediatr Onco135:651-655
DNA and mRNA products were about the same, we chose to exclude these samples from further analysis.
In summary, we found that both the commercially available kits from Roche
Diagnostics and our own protocol give reproducible and accurate results. However, for better reproducibility and comparability between results obtained in different laboratories, we recommend using the standardized kits.
References
1. Shay JW, Bacchetti S (1997) A survey of telomerase activity in human cancer. Eur J Cancer
33:787-791
2. Healy KC (1995) Telomere dynamics and telomerase activation in tumor progression:
prospects for prognosis and therapy. Oncol Res 7:121-130
3. Poremba C, Bocker W, Willenbring H, Schafer KL, Otterbach F, Burger H et al. (1998) Telomerase activity in human proliferative breast lesions. Int J Oncol12:641-648
4. Poremba C, Shroyer KR, Frost M, Diallo R, Fogt F, Schafer K et al. (1999) Telomerase is a highly sensitive and specific molecular marker in fine-needle aspirates of breast lesions. J Clin
Oncol17:2020-2026
5. Poremba C, Willenbring H, Hero B, Christiansen H, Schafer KL, Brinkschmidt C et al. (1999)
Telomerase activity distinguishes between neuroblastomas with good and poor prognosis.
Ann OncollO:715-721
6. Morin GB (1989) The human telomere terminal transferase enzyme is a ribonucleoprotein
that synthesizes TTAGGG repeats. Cell 59:521-529
7. Ramakrishnan S, Eppenberger U, Mueller H, Shinkai Y, Narayanan R (1998) Expression profile of the putative catalytic subunit of the telomerase gene. Cancer Res 58:622-625
8. Avilion AA, Piatyszek MA, Gupta J, Shay JW, Bacchetti S, Greider CW (1996) Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. Cancer Res
56:645-650
9. Ito H, Kyo S, Kanaya T, Takakura M, Inoue M, Namiki M (1998) Expression of human telomerase subunits and correlation with telomerase activity in urothelial cancer. Clin Cancer Res
4: 1603-1608
10. Poremba C, Scheel C, Hero B, Christiansen H, Schaefer KL, Nakayama J et a1. (2000) Telomerase activity and telomerase subunits gene expression patterns in neuroblastoma: a molecular and immunohistochemical study establishing prognostic tools for fresh-frozen and
paraffin-embedded tissues. J Clin Oncol18:2582-2592
11. Poremba C, Hero B, Heine B, Scheel C, Schaefer KL, Christiansen H et al. (2000) Telomerase
is a strong indicator for assessing the proneness to progression in neuroblastomas. Med
Pediatr Onco135:651-655