IIl9I Applications in Oncology
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Fig.4a,b. Real-time RT-PCR using the LightCyder method of the house-keeping gene TBP. a The
amplification curves of different amounts of the TBP RT -PCR product. These results were used
as standard curves for the quantification of TBP mRNA expression. b The regression analysis of
these curves (r=O.98)
patients or specific vaccination strategies with peptide-pulsed dendritic cells in
melanoma. Efficient specific vaccination strategies depend on the definition of
adequate target structures recognized by cytotoxic and helper T lymphocytes
resulting in cellular or humoral immune responses. The tumor-associated antigen
PRAME is expressed at a high level in different solid tumors, but also in hematological malignancies like acute myeloid leukemia. PRAME was the first tumorassociated cancer/testis antigen with high mRNA expression detected in leukemia