II'D Applications in Oncology
The peR protocol was:
Parameter
Value
Cycles
45
Type
Quantification
Segment I
Segment 2
Segment 3
Target tempe rature [0C]
95
64
72
Incubatjon time [5)
10
10
26
Temperature transition rate [OC/s)
20
20
2
Acqillsition mode
None
None
Single
Gains
FI= I ; F2= 15
Table 1. Oligonucleotides
AMLI-ETO (GcnBank Accession I1D13979
Position EXOD
Length GC(%) Tm(OC)
Primers
FS'GAGGGAAAAGCTTCACTCTG3'
2.003
4
20
50
60.7
RS'TCGGGTGAAKIGTCATTGCC3'
2,450
3
20
50
63.5
Probes
5'CCCTCACCACCCAATGGCTTCAGC-F
2,293
3
24
62.5
73.3
5'LCRED640-TGGGCCTTCCTCTTCTTCC
2.319
3
25
60
72.6
TCCTCC-P
G6PDH (GenBank Accession I1XS5448)
Primers
5'CCGGATCGACCACIACCTGGGCAAG-3'
15.116
G6PDH 6
25
64
73.8
5'GTTCCCCACGTACTGGCCCAGGACCA-3' 16.44.3R G6PDH 9
26
65
76.8
Probes
5'GTTCCAGATGGGGCCGAAGATCCT
15.376R G6PDH 7
28
57
72.7
GTTG-F
5' -LCRED640-CAAATCTCAGCACCAT
15.165R G6PDH 7/6 28
50
70.0
GAGGTTCTGCAC-P
cABL (GenBank Accession I1U07563)
Primers
5'CCCAACCTITTCGTTGCACTGT3'
49.996
ABla2
22
SO
66.5
5'CGGCTCTCGGAGGAGACGTAGA3'
58.610R
ABLa4
22
64
69.3
Probes
5'TGAAAAGCTCCGGGTCTTAGGCTA
50.627
ABLa3
30
43
70.1
TAATCA-F
5' -LCRED640-AATGGGGAATGGTGTG
50,658
ABLa3
25
48
70.7
AAGCCCAAA-P