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Applications in Oncology
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Fig. 2. Two color multiplexing of HPV 16, HPV 18 and HPV 45 in a single capillary. The starting
concentration of each subtype was approximately 10 5 copies/f!!. All three subtypes were amplified in a background of SOng/f!! of human genomic DNA. (a) The HPV 18 and HPV 45 products
were detected in channel 2 and differentiated by melting temperature. (b) HPV 16 was characterized by color in channel 3
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