A
C
" .. .. . .
0
u
;fThe Heterotrimeric G Protein Genes of Caenorhabditis elegans
23
Na Ac YS NoCI
B
glucose vs ..
Na Ac
1.00
" "
"
.S
. .
';(
to
;;
0.00
E
"
~
"
_ _ w ~dlype
NoCI
_ _ gpa·3
·0.60
10
20
30
40
50
60
90
10
lime (min)
0.45
0.35
0.25
0.15
0.05
O ·~~~AW~~~~~~~~~~~
20
30
40
50
60
lime (min)
o 1.0
><
' "
" ,!; . . 0.5
.;
. .
;;
E
"
r.
"
Iml
laa
Fig. SA-D. Six Ga knockout alleles show chemotaxis defects. A,B, gpa-3
seems involved in chemotaxis to water soluble compounds. Mutant or wildtype animals are given a choice between NaAc or NaCI (A), or are tested for
chemotaxis to glucose (B). The chemotaxis index in time is plotted. A chemotaxis index of 1 means complete attraction to NaAc (A) or glucose (B),
whereas -1 means complete attraction to NaCI (A) or avoidance of glucose
(B). C gpa-3, -7, and odr-3 seem involved in water-soluble chemoaversion.
Animals were tested for avoidance of 150 mM and 15 mM CUS04, 4% and
0.4% SDS, and 4 M fructose. The percentages of animals that crossed the aversive barrier, attracted by isoamylalcohol, are plotted for each mutant. D gpa-5
is a negative regulator of 2,4,5-trimethylthiazole (tmt) perception. The chemotaxis index is plotted for 100 times diluted tmt and isoamyl alcohol (iaa)
glucose