24
G. Jansen et al.
Table 2. Summary of behavioral assays
Strain
Water soluble chemoattraction a
Water soluble chemoaversion b
Ac-CI
NaCI
NaAc
Glucose 150
15
osm
4%SDS
CUS04
CUS04
gpa-l
wt
nd
nd
wt(4)
wt
wt
wt
wt
wt
gpa-1XS Ac(4)*
mut*
mut*
wt
mut*
mut*
mut*
mut*
mut*
gpa-2
wt(4)
nd
nd
wt(4)
wt(6)
wt(6)
wt(5)
wt
wt
gpa-2QL wt
nd
nd
wt
wt
wt
wt
wt
wt
gpa-3
CI(4)*
mut(4)* mut(4)* mut(8)* mut(4)* mut(4)* mut(8)* wt
mut*
gpa-3QL CI*
mut*
mut
mut*
wt
wt
wt
nd
nd
gpa-4
wt
nd
nd
wt(6)
wt
wt
wt
wt
wt
gpa-4XS Ac(4)*
mut(4)* mut(4)* wt(4)
wt
wt
wt
wt
wt
gpa-S
wt
nd
nd
wt(4)
wt
wt
wt
wt
wt(4)
gpa-SXS wt(4)
nd
nd
wt(4)
wt
wt
wt
wt
wt
gpa-6
CI(6)*
wt
wt
wt
wt
wt
wt
wt
wt
gpa-6XS Ac(6)
wt
wt
wt
wt
wt(4)
wt
wt
wt
gpa-7
wt(4)
nd
nd
wt
wt
wt
wt
wt
mut*
gpa-7XS CI(4)*
mut(4)* wt(4)
mut(4)* wt
wt
mut(4)* mut(4)* mut(4)*
gpa-8
wt
nd
nd
wt(4)
wt
wt
wt(4)
wt
wt
gpa-8XS wt(6)
wt(4)
wt
wt(4)
wt
wt
wt
wt
wt
gpa-9
wt(4)
nd
nd
wt(8)
wt
wt(4)
wt(6)
wt
wt
gpa-IO
wt
nd
nd
wt
wt
wt
wt
wt
wt
gpa-JOXS CI(4)*
mut(4)* mut(4)* mut(4)* mut*
mut
mut*
mut*
mut*
gpa-ll
wt
nd
nd
wt
wt
wt
wt
wt
wt(4)
gpa-IIXS wt(4)
wt
wt
mut(6)* wt
wt
wt
wt
wt(4)
gpa-14
wt
wt
wt
wt
wt
wt
wt
wt
wt
gpa-IS
wt(6)
nd
nd
wt(4)
wt
wt
wt
wt
wt(4)
odr-3
wt
nd
nd
mut(4)* mut(4)* mut*
mut*
mut(4)
nd
gpa-S,
nd
nd
nd
nd
nd
nd
nd
nd
nd
odr-3
The response of all G protein mutants in various behavioral assays is given. Assays that showed altered
behavior are in bold. All assays were performed twice, unless otherwise indicated (n). Some odr-3
phenotypes have been reported previously (Roayaie et al. 1998).
wt, wild type behavior; mut, reduced chemotaxis to the attractant, or reduced avoidance; nd, not determined.
*Mutants that showed significantly altered behavior (P.05).
aChemotaxis to water soluble compounds. Ac-CI, animals were presented with a choice between NaAc and
NaCI. Increased preference for Ac (Ac), or Cl (Cl) is indicated. Chemotaxis to NaCI, NaAc, and glucose.
b Aversion of water soluble compounds, 150 mM or 15 mM CUS04; 4 M fructose (osm); 4% SDS, 0.4%
SDS.
CChemotaxis to volatile attractants, trimethylthiazole (tmt), diacetyl (dia), pyrazine (pyr) (10 mg/ml).
isoamyl alcohol (iaa), benzaldehyde (ben), pentanedione (pen). All odorants were diluted 100 times in
ethanol, unless otherwise indicated.
d Avoidance of volatile compounds, non, 2-nonanone; oct, l-octanol, undiluted. Assays performed using 10
times diluted odorants confirmed the results.
e gpa-I XS and gpa-Il XS animals showed reduced potency (55% and 40% potency. respectively), 80-100%
of the males of all other strains were potent.
f Animals were incubated with the fluorescent dye DiO, filling of amphid and phasmid neurons was tested.
gpa-10XS animals showed stronger staining, gpa-3QL animals showed no filling.