370
post-translational modification 21
- acetylation 258
- dephosphorylation 258
- micro-deglycosylation 40
- mLlMS/MS 10
- phosphorylation 9, 10, 23, 24, 258
- S-pyridylethylated peptides 51
- spectra 10
potato carboxypeptidase inhibitor 189, 190
primer 66
progelatinase 293
proline, cis/trans isomers 199-208
promotor, lac-z 274
protease
- inhibitor 352
- membrane-bound 291
protein 24, 218
- carbohydrate-binding 241
- carboxymethylation 191
- composition 241
- concentration 220
- conformation 238
- crosslin king 241
- deblocking 85
- denaturated 191,218, 283
- detection on polyacrylamide gels, reversestaining 31-33
- digestion 40, 41
- - buffers 40
- electrostatic interactions 211
- FO-protein 127
- fast passive elution 37, 38
folding (see there)
- gel separated, characterisation 81-86
- G-protein 131
- hydrophobic interactions 211, 212
- identification 7, 8, 54
- - database searching 59
- - in mixtures 54
- in-gel digestion 45, 50, 81-84
- layers 238
- low abundance, challenges 8, 9
- membrane 243
- mobilisation/alkylation 33, 34
- molecular weight 182
- N-terminally modified, deblocking 303-317
- native 180, 192
- native structure 211
- negative staining, reversible 29-52
- nucleoprotein 260
- phosphoprotein 257
- plasma 243-245
- post-translational modified 21,258
- primary structure 244
- recombinant 175
- reduced 191
- renaturated 283
- S 14 ribosomal protein, Zn containing
273-286
- saposin-like 251-254
- secondary modifications, characterization
21-24
Subject Index
- seminal fluid 244
- sequence 4, 107
- sequencing 24
- - de novo 65-78
- - peptide maps 21
- - sequencing column 38
- single domain 189
- structure 246, 274
- succinylated 182
- TthS14 282
protein complexes, direct analysis
- applications 62, 63
- introduction 53
- identification in mixtures 54
- sofhvare 55-59
protein structure/dynamics by NMR in solution
- introduction 91, 92
- sample requirements 92-94
protein-protein-contact 237
proteoglycans 289
- biological roles/strategies for isolation
341-361
- labelling, isolation, characterisation
350-356
- protein core 348, 349
- structure 349, 350
proteolysis 336
- experiments 279, 280
proteome 3, 24
- analysis 3, 4, 30
- - identification, protein 17, 59
- - mass spectroscopy 44-51
- - separation 16
- - visualization 16
- project 4
- sequencing 4
proteomics 51
proton translocation 225, 226
Psp I/Psp II (porcine seminal plasma)
241-249
- crystal structure 245-247
PTH 84,314
purification, ammonium sulfate 8
PVDF-disc, chromatographic concentration
38,39
Pyroccocus furiosus 304
pyroglutamic acid, removing 310
pyroglutamyl peptidase 310
R
rabbit 241
radioactivity 352
Raman spectrum 236, 237
rat 241
reduction, thiol reagents 34
refolding 191,200
renaturation 283
- in-gel 36
respiratory syncytial virus (RSV) 127-133
retinal
- analogues 230
- spectrum 236
post-translational modification 21
- acetylation 258
- dephosphorylation 258
- micro-deglycosylation 40
- mLlMS/MS 10
- phosphorylation 9, 10, 23, 24, 258
- S-pyridylethylated peptides 51
- spectra 10
potato carboxypeptidase inhibitor 189, 190
primer 66
progelatinase 293
proline, cis/trans isomers 199-208
promotor, lac-z 274
protease
- inhibitor 352
- membrane-bound 291
protein 24, 218
- carbohydrate-binding 241
- carboxymethylation 191
- composition 241
- concentration 220
- conformation 238
- crosslin king 241
- deblocking 85
- denaturated 191,218, 283
- detection on polyacrylamide gels, reversestaining 31-33
- digestion 40, 41
- - buffers 40
- electrostatic interactions 211
- FO-protein 127
- fast passive elution 37, 38
folding (see there)
- gel separated, characterisation 81-86
- G-protein 131
- hydrophobic interactions 211, 212
- identification 7, 8, 54
- - database searching 59
- - in mixtures 54
- in-gel digestion 45, 50, 81-84
- layers 238
- low abundance, challenges 8, 9
- membrane 243
- mobilisation/alkylation 33, 34
- molecular weight 182
- N-terminally modified, deblocking 303-317
- native 180, 192
- native structure 211
- negative staining, reversible 29-52
- nucleoprotein 260
- phosphoprotein 257
- plasma 243-245
- post-translational modified 21,258
- primary structure 244
- recombinant 175
- reduced 191
- renaturated 283
- S 14 ribosomal protein, Zn containing
273-286
- saposin-like 251-254
- secondary modifications, characterization
21-24
Subject Index
- seminal fluid 244
- sequence 4, 107
- sequencing 24
- - de novo 65-78
- - peptide maps 21
- - sequencing column 38
- single domain 189
- structure 246, 274
- succinylated 182
- TthS14 282
protein complexes, direct analysis
- applications 62, 63
- introduction 53
- identification in mixtures 54
- sofhvare 55-59
protein structure/dynamics by NMR in solution
- introduction 91, 92
- sample requirements 92-94
protein-protein-contact 237
proteoglycans 289
- biological roles/strategies for isolation
341-361
- labelling, isolation, characterisation
350-356
- protein core 348, 349
- structure 349, 350
proteolysis 336
- experiments 279, 280
proteome 3, 24
- analysis 3, 4, 30
- - identification, protein 17, 59
- - mass spectroscopy 44-51
- - separation 16
- - visualization 16
- project 4
- sequencing 4
proteomics 51
proton translocation 225, 226
Psp I/Psp II (porcine seminal plasma)
241-249
- crystal structure 245-247
PTH 84,314
purification, ammonium sulfate 8
PVDF-disc, chromatographic concentration
38,39
Pyroccocus furiosus 304
pyroglutamic acid, removing 310
pyroglutamyl peptidase 310
R
rabbit 241
radioactivity 352
Raman spectrum 236, 237
rat 241
reduction, thiol reagents 34
refolding 191,200
renaturation 283
- in-gel 36
respiratory syncytial virus (RSV) 127-133
retinal
- analogues 230
- spectrum 236