Subject Index
ribonuclease A 197
ribosomal proteins
- SI4 284
- S27 284
- structure 273
- zinc-finger 283
ribosomes 62, 63, 273, 276
- rat 273
RNA 273
- 16S rRNA 273
rotational diffusion 102, 103, 110
- anisotropy 103
S
SI4 ribosomal protein, Zn containing 273
- cloning 274
- overproduction 274-276
- purification 276-279
- structural studies 279-286
sample
- crystallization 139
- introduction, mass spectroscopy 6, 7
- preparation, mass spectroscopy 5, 42
saposin-like protein 251-254
- analysis 252, 253
Schiff base 229, 234
SDS-polyacrylamide gels 29
- protein detection 31, 32
secondary structure, protein 96
seminal plasma, porcine (Psp I/Psp II)
241-249
separation
- global protein separation 5
- multi-dimensional 61, 62
sequence 259
- comparison 251
- PyPu 260
- recognition 264
sequence analysis 3, 81, 84
- Procise cLc 84
sequencing
- amino acid 81
- amino-terminal 279
- automatic 183
- CID-linked 180
- protein 16, 24
de novo 65
- - abstract 65
- - introduction 66, 67
- - methods 67
- DNA (see there)
- Edman sequencing 127, 185, 196
- internal 7
- N-terminal 7
- yields 314
serglycin 350
Sepadex G-25 214
single band transfer 35
spectra
- CD 141,220,221,223
- NOESY 109
- PSD 122
- UV 51
spectroscopy
- CD 144
- FTIR-difference 237
- infrared 226-229
- mass spectroscopy (see there)
- optical 231
- UV-Vis 238
- vibrational 235-237
sperm protein 241
spermadhesin 241, 244-249
- overview 242-244
staining, reversible negative
- introduction 29, 30
- double staining, Coomassie-blue 32, 33
stationary phases,cyclodextrin bonded
201-204
structure 241
- j3-barrel!j3.-pleated 293
- 3-D structure 91, 96, 297, 303
- chemical shift mapping 96
- domain 291
- primary 303
- secondary 217,222
- tertiary 217
sugar 321,325,326,328,336
Sulfolobus sulfataricus 286
Superose 6 355
surfactant, lung 251
T
tetrapeptide 203
tetrasaccharid 341
371
Thermus thermophilus 273,274,281,283-285
tick anticoagulat peptide (TAP) 189
- folding intermediates 191-194
TIMPs 289-298
titration 219
TOF (time of flight) 72-77, 191
- quadrupole- (Q-TOF) 21 , 117
translocation 199, 234
tripellhelicase 292
trypsin 19, 50, 61, 81
tryptophyllin 3201
TthSI4, atomic absorption 281
tumor 290
- suppression 257
u
unfolding 191
- thermal 215, 216, 218
V
virus 330
vitronectin 289
X
X-ray 91,291,297,321
- crystallography 293
- diffraction 229-235
- - time-resolved 231
ribonuclease A 197
ribosomal proteins
- SI4 284
- S27 284
- structure 273
- zinc-finger 283
ribosomes 62, 63, 273, 276
- rat 273
RNA 273
- 16S rRNA 273
rotational diffusion 102, 103, 110
- anisotropy 103
S
SI4 ribosomal protein, Zn containing 273
- cloning 274
- overproduction 274-276
- purification 276-279
- structural studies 279-286
sample
- crystallization 139
- introduction, mass spectroscopy 6, 7
- preparation, mass spectroscopy 5, 42
saposin-like protein 251-254
- analysis 252, 253
Schiff base 229, 234
SDS-polyacrylamide gels 29
- protein detection 31, 32
secondary structure, protein 96
seminal plasma, porcine (Psp I/Psp II)
241-249
separation
- global protein separation 5
- multi-dimensional 61, 62
sequence 259
- comparison 251
- PyPu 260
- recognition 264
sequence analysis 3, 81, 84
- Procise cLc 84
sequencing
- amino acid 81
- amino-terminal 279
- automatic 183
- CID-linked 180
- protein 16, 24
de novo 65
- - abstract 65
- - introduction 66, 67
- - methods 67
- DNA (see there)
- Edman sequencing 127, 185, 196
- internal 7
- N-terminal 7
- yields 314
serglycin 350
Sepadex G-25 214
single band transfer 35
spectra
- CD 141,220,221,223
- NOESY 109
- PSD 122
- UV 51
spectroscopy
- CD 144
- FTIR-difference 237
- infrared 226-229
- mass spectroscopy (see there)
- optical 231
- UV-Vis 238
- vibrational 235-237
sperm protein 241
spermadhesin 241, 244-249
- overview 242-244
staining, reversible negative
- introduction 29, 30
- double staining, Coomassie-blue 32, 33
stationary phases,cyclodextrin bonded
201-204
structure 241
- j3-barrel!j3.-pleated 293
- 3-D structure 91, 96, 297, 303
- chemical shift mapping 96
- domain 291
- primary 303
- secondary 217,222
- tertiary 217
sugar 321,325,326,328,336
Sulfolobus sulfataricus 286
Superose 6 355
surfactant, lung 251
T
tetrapeptide 203
tetrasaccharid 341
371
Thermus thermophilus 273,274,281,283-285
tick anticoagulat peptide (TAP) 189
- folding intermediates 191-194
TIMPs 289-298
titration 219
TOF (time of flight) 72-77, 191
- quadrupole- (Q-TOF) 21 , 117
translocation 199, 234
tripellhelicase 292
trypsin 19, 50, 61, 81
tryptophyllin 3201
TthSI4, atomic absorption 281
tumor 290
- suppression 257
u
unfolding 191
- thermal 215, 216, 218
V
virus 330
vitronectin 289
X
X-ray 91,291,297,321
- crystallography 293
- diffraction 229-235
- - time-resolved 231