368
identification, amide groups 189
IgG 151, 152
illumination 234
immune response 333
immunity 337
irnmunsuppressiva 201
inhibition, TIMPs 289
inhibitors 294, 297
insect, immunity 157
interaction
- bimolecular 292-298
- C-terminal 296
- hydrophobic 211-213,215,217,351
intermediate state, characterisation
226-229
ion-exchange 354
isomerase 199
isomerization, cis/trans 199-202
isotopic labeling 69, 94
J
jellyfish 171
K
Karplus relationship 97
kinetic methods 200
Kunitz-type protease inhibitor 189
L
a-lactalbumin 211-214,216,218
- bovine 213
- conformation 212
Laemmli running buffer 37
laminin 289
lectin 241, 248
lectin-carbohydrate binding 241, 242
leukocyte 330
ligand, pellucida 241
lipid
- bilayer 235
- chain 330
lyase 361
lysine 180
- blocked 185
- NK-lysin, recombinant 253
M
MALDI (matrix-assisted laser desorption/
ionisation) 6, 13, 16, 17, 19,21,42,81,83,
117, 191, 197
- specific zinc fmger peptide complexes,
detection 139-146
MALDI-TOF MS 45-47, 117-136
- defense reaction in Drosophila meianogaster
157-172
- - complex mixtures, critical points 159
- - DIMs
- identification, sequencing 169-171
- - - induction 168, 169
- - - molecular mass characterization
163-166
Subject Index
- - - time course of induction/ degradation
166, 167
- - drosomycin gene, inducible tissue-specific
expression 171, 172
hemolymph, differential analysis 161-163
- - sample preparation 160, 161
molecular analyses 120, 121
- post-source decay analyses 122-124
maleic anhydride 185
mannose 245
mass fingerprint 45
mass spectra, database 54
mass spectroscopy 24, 25, 175
- accuracy 16
- CID-MS 83
- data analysis 60
- differential scanning 77, 78
ESI-MS/MS 6,7, 13, 16, 17, 19,21,42,81,83
- FAB 180, 185
- FT-ICR 13
- glycan structure 22
- glycoproftle 22
- hybrid structure 22
- in-source CID 11, 12
- LC/MS 43, 59
- MALDI-TOF MS (see there)
- mannose 22
- mapping 19
- MS/MS/MS 11
- matrix-assisted laser desorption/ionisation
(see MALDI)
- nanospray 19
- nitrocellulose 18
- non-phosphopeptide 12
- phosphopeptide 12
- peptide signals 19
- post -source decay 117
- protein mixture analysis 60, 61
- PSD 135, 136
- time of flight (see TOF)
- sample introduction 6, 7
- sample preparation 5
- sensitivity 16
- synthetic peptide 118-120
- tandem 55, 59, 66, 82
- - software 55-59
- target 18
- triple quadrupole 68, 78
membrane
- protein 225, 229
- PVDF 84
membranes
- Fluorotrans 307
Hyperbond 307
Immobilon-P 307
Problott 307
purple 237
PVDF 304,317
- teflon-membran 307
- Transblott 307
metalloproteinase, bimolecular interactions
289-298
identification, amide groups 189
IgG 151, 152
illumination 234
immune response 333
immunity 337
irnmunsuppressiva 201
inhibition, TIMPs 289
inhibitors 294, 297
insect, immunity 157
interaction
- bimolecular 292-298
- C-terminal 296
- hydrophobic 211-213,215,217,351
intermediate state, characterisation
226-229
ion-exchange 354
isomerase 199
isomerization, cis/trans 199-202
isotopic labeling 69, 94
J
jellyfish 171
K
Karplus relationship 97
kinetic methods 200
Kunitz-type protease inhibitor 189
L
a-lactalbumin 211-214,216,218
- bovine 213
- conformation 212
Laemmli running buffer 37
laminin 289
lectin 241, 248
lectin-carbohydrate binding 241, 242
leukocyte 330
ligand, pellucida 241
lipid
- bilayer 235
- chain 330
lyase 361
lysine 180
- blocked 185
- NK-lysin, recombinant 253
M
MALDI (matrix-assisted laser desorption/
ionisation) 6, 13, 16, 17, 19,21,42,81,83,
117, 191, 197
- specific zinc fmger peptide complexes,
detection 139-146
MALDI-TOF MS 45-47, 117-136
- defense reaction in Drosophila meianogaster
157-172
- - complex mixtures, critical points 159
- - DIMs
- identification, sequencing 169-171
- - - induction 168, 169
- - - molecular mass characterization
163-166
Subject Index
- - - time course of induction/ degradation
166, 167
- - drosomycin gene, inducible tissue-specific
expression 171, 172
hemolymph, differential analysis 161-163
- - sample preparation 160, 161
molecular analyses 120, 121
- post-source decay analyses 122-124
maleic anhydride 185
mannose 245
mass fingerprint 45
mass spectra, database 54
mass spectroscopy 24, 25, 175
- accuracy 16
- CID-MS 83
- data analysis 60
- differential scanning 77, 78
ESI-MS/MS 6,7, 13, 16, 17, 19,21,42,81,83
- FAB 180, 185
- FT-ICR 13
- glycan structure 22
- glycoproftle 22
- hybrid structure 22
- in-source CID 11, 12
- LC/MS 43, 59
- MALDI-TOF MS (see there)
- mannose 22
- mapping 19
- MS/MS/MS 11
- matrix-assisted laser desorption/ionisation
(see MALDI)
- nanospray 19
- nitrocellulose 18
- non-phosphopeptide 12
- phosphopeptide 12
- peptide signals 19
- post -source decay 117
- protein mixture analysis 60, 61
- PSD 135, 136
- time of flight (see TOF)
- sample introduction 6, 7
- sample preparation 5
- sensitivity 16
- synthetic peptide 118-120
- tandem 55, 59, 66, 82
- - software 55-59
- target 18
- triple quadrupole 68, 78
membrane
- protein 225, 229
- PVDF 84
membranes
- Fluorotrans 307
Hyperbond 307
Immobilon-P 307
Problott 307
purple 237
PVDF 304,317
- teflon-membran 307
- Transblott 307
metalloproteinase, bimolecular interactions
289-298