Subject Index
Edman sequencing 127, 185, 196
elastin 289
electroblotting 85, 86
- PVDF membranes 85, 86
electrophoresis
- capillary zone (CZE) 207, 208
- gel
- - 2-D 8, 13,62,81,83,85,86, 172,304,314
- - diagonal 176
- - extraction 38
- - hydrophobic proteins 37
- - in-gel renaturation 36
- - polyacrylamide gel electrophoresis (see
PAGE)
- paper 176
- - protein detection 30
- - reversible negative staining 29-52
- - separated proteins, characterization
81-86
- - silver staining 8
ELISA 151-153
elution, octyl-Sepharose 356
entactin 289
Entamoeba histolytica 286
enzyme 292,297,324
epitope mapping, monoclonal antibody
149-154
erythrocytes 330
Escherichia coli 253, 274, 276
extraction, ZIP-Tips 6
F
Fab 151
fast atom bombardment 117
fibromodulin 347
fibronectin 289
fibroplast 291
fibrosis 289, 290
fixation 34
flavin 150
flavocytochrome b2 149
- crystal structure 149
- heme binding domain 149, 153, 154
- mutation 152, 153
- - chemical 153, 154
- 3-D structure 150
fluorescence
- labelling 296, 305
- measurement 214, 219, 220
- tryptophan 223
fluorescence energy transfer 222, 223
fluorimeter 214
folding experiments 190, 191
folding pathway, disulfide containing proteins
189-197
FTIR 238
- measurement 233
- spectroscopy 237
fungi 93, 330
G
galactosamine 346
galectin 248
gametes 241
gel blotting 35
gelatin 289
gelatinase 292, 293
genome 15, 25
- sequencing 53
global hydrodynamics 109
glucose 245, 323, 334, 336
glutathione, reduced 192
glycan 133, 241, 248, 325, 330-333, 341
- isolation, characterisation 356, 357
- sequencing 322-324
glycoconjugates, structural diversity/
importance 341-343
367
glycoprotein 241,242, 245, 248, 249, 321, 324,
336, 342, 343
glycosaminoglycans 344
- characterisation 358
- isolation 357, 358
glycosilation 321, 333, 334, 336
- mannose 342
guinea pig 241
H
Halobacterium salinarum 225
helix
- a-helix 229
- II 113
- C-terminal 291
heme b2 149, 150, 153
heparin 245, 347, 361
heparin lyase II 361
heparinase 361
hexosamine 347
hirudin 189-197
- folding intermediates 191-194
HIV, antiviral agents 146
hormone 341
host defence, glycosilation 321-337
HPLC 157, 170, 191, 194,200,322-324,354,
358, 361
- cavity supported, cs/trans isomers of
proline 199-208
- ion-pair 360
- microcolumn 48, 179
- multi-dimensional 61
- peptide mapping 176
- proline, cis/trans isomers 200-207
- reversed-phase capillary analysis 23, 48-51,
61, 62, 84, 120, 180, 182, 184,200
- RP 18 205, 206
- RP-C4-column 178
- UV-detection 43, 133
human 241
human epidermal growth factor 189, 190
hyaluronan 350
Edman sequencing 127, 185, 196
elastin 289
electroblotting 85, 86
- PVDF membranes 85, 86
electrophoresis
- capillary zone (CZE) 207, 208
- gel
- - 2-D 8, 13,62,81,83,85,86, 172,304,314
- - diagonal 176
- - extraction 38
- - hydrophobic proteins 37
- - in-gel renaturation 36
- - polyacrylamide gel electrophoresis (see
PAGE)
- paper 176
- - protein detection 30
- - reversible negative staining 29-52
- - separated proteins, characterization
81-86
- - silver staining 8
ELISA 151-153
elution, octyl-Sepharose 356
entactin 289
Entamoeba histolytica 286
enzyme 292,297,324
epitope mapping, monoclonal antibody
149-154
erythrocytes 330
Escherichia coli 253, 274, 276
extraction, ZIP-Tips 6
F
Fab 151
fast atom bombardment 117
fibromodulin 347
fibronectin 289
fibroplast 291
fibrosis 289, 290
fixation 34
flavin 150
flavocytochrome b2 149
- crystal structure 149
- heme binding domain 149, 153, 154
- mutation 152, 153
- - chemical 153, 154
- 3-D structure 150
fluorescence
- labelling 296, 305
- measurement 214, 219, 220
- tryptophan 223
fluorescence energy transfer 222, 223
fluorimeter 214
folding experiments 190, 191
folding pathway, disulfide containing proteins
189-197
FTIR 238
- measurement 233
- spectroscopy 237
fungi 93, 330
G
galactosamine 346
galectin 248
gametes 241
gel blotting 35
gelatin 289
gelatinase 292, 293
genome 15, 25
- sequencing 53
global hydrodynamics 109
glucose 245, 323, 334, 336
glutathione, reduced 192
glycan 133, 241, 248, 325, 330-333, 341
- isolation, characterisation 356, 357
- sequencing 322-324
glycoconjugates, structural diversity/
importance 341-343
367
glycoprotein 241,242, 245, 248, 249, 321, 324,
336, 342, 343
glycosaminoglycans 344
- characterisation 358
- isolation 357, 358
glycosilation 321, 333, 334, 336
- mannose 342
guinea pig 241
H
Halobacterium salinarum 225
helix
- a-helix 229
- II 113
- C-terminal 291
heme b2 149, 150, 153
heparin 245, 347, 361
heparin lyase II 361
heparinase 361
hexosamine 347
hirudin 189-197
- folding intermediates 191-194
HIV, antiviral agents 146
hormone 341
host defence, glycosilation 321-337
HPLC 157, 170, 191, 194,200,322-324,354,
358, 361
- cavity supported, cs/trans isomers of
proline 199-208
- ion-pair 360
- microcolumn 48, 179
- multi-dimensional 61
- peptide mapping 176
- proline, cis/trans isomers 200-207
- reversed-phase capillary analysis 23, 48-51,
61, 62, 84, 120, 180, 182, 184,200
- RP 18 205, 206
- RP-C4-column 178
- UV-detection 43, 133
human 241
human epidermal growth factor 189, 190
hyaluronan 350