366
columns
- chromatographic concentration 38, 39
complement control protein (CCP) 330
complement pathway, inhibitors 330-333
complex 139
- formation 296
- glycan 336
- tetrameric 261-264
conformation, conformational change 218,
220-222
- charge controlled 234, 235
conservation
- evolutionary 267
- p53DBD 267
Coomassie-blue (CB) 32, 33
cross-linking 273
crystal structure 149, 245-247
crystalization, lipidic cubic phase 236
C-terminal peptide 66, 107, 180, 185,253, 295
- isolation 175-187
- labeling 66
C-terminal fragment 65, 122, 237, 264
cyclodextrin 199-208
- carboxymethylated 208
cyclosporin A 201
cysteine 192, 276
cytochrome
- b2 149, 153
- C/C2 109-113, 150, 184, 185
- - local motion/overall tumbling 110, 111
- - rotational tumblinglbackbone dynamics
109, 110
- - structural/dynamic information,
comparison 111-113
cytoplasmic side 234, 235
D
dabsyl chloride 191
data analysis, post search 60
data processing 68, 69
databank, Brookhaven protein databank (pdb)
91
database 19
- BLAST 84, 286
- EST 17,78
- mass spectra 54, 59, 66
- nucleic acid 82
- protein 82
- sequence homology 84
- sequence search 8
- sequest 8
deblocking aminopeptidase (DAP) 313
deblocking, proteins
- chemical
- - alcoholic deacetylation 315
- - N -terminal acetylserine/acetylthreonine
313,315
- - N-terminal pyroglutamic acid 315
- enzymatic 304
- - acylamino acid releasing enzyme 311-313
- - aminopeptidase 304-310
- - N-terminal pyroglutamic acid 310
- sample preparation 317
- sequential 319,317
deglycosilation, micro- 40
dermorphin 201
desalting 177
destaining 83
dextran 324
dichroism, circular 213,215, 216
Subject Index
differential scanning technique 65, 69, 77, 78
digestion 93
- buffer 40
- enzymatic 66, 354
- in -gel 45, 50, 81-85
- in-situ 21
- peptide 18,51,55
- protease 333
- protein 61, 177
- tryptic fragments 83, 180
dipolar contribution 103
disaccharide 343, 346, 347
- o-disaccharides 359, 361
disulfide 194, 196, 197
- bond, MALDI-TOF-MS 131
- bridges 247
- folding 190
- hydrophobic interactions 211,212
- native bonds 189
- scrambled 192
DITC 317
DNA
- binding properties 257
- nucleosomal 268
- sequencing 25,81, 175
- - cDNA 15,24
- - database 15
- - genomic 16
domains 247, 333
- C-terminal 293
- C-terminal-trans membrane 292
- catalytic 295-297
- CCP 337
- CH2 326
- extracellular 332
- functional 258
- heme binding 149, 153, 154
- hydrophobic 218
- saposin 252, 253
- single 189
Drosophila melanogaster
- antimicrobial peptides 158
- defense reaction 157 -172
- genome 170
- hemolymph 161-163, 168, 171
- immunity 158
- morphological level 158
- septic injury 158
- sequence 84
E
ECO RI 151
Edman degradation 24, 85, 86, 175, 185, 303,
313,317
columns
- chromatographic concentration 38, 39
complement control protein (CCP) 330
complement pathway, inhibitors 330-333
complex 139
- formation 296
- glycan 336
- tetrameric 261-264
conformation, conformational change 218,
220-222
- charge controlled 234, 235
conservation
- evolutionary 267
- p53DBD 267
Coomassie-blue (CB) 32, 33
cross-linking 273
crystal structure 149, 245-247
crystalization, lipidic cubic phase 236
C-terminal peptide 66, 107, 180, 185,253, 295
- isolation 175-187
- labeling 66
C-terminal fragment 65, 122, 237, 264
cyclodextrin 199-208
- carboxymethylated 208
cyclosporin A 201
cysteine 192, 276
cytochrome
- b2 149, 153
- C/C2 109-113, 150, 184, 185
- - local motion/overall tumbling 110, 111
- - rotational tumblinglbackbone dynamics
109, 110
- - structural/dynamic information,
comparison 111-113
cytoplasmic side 234, 235
D
dabsyl chloride 191
data analysis, post search 60
data processing 68, 69
databank, Brookhaven protein databank (pdb)
91
database 19
- BLAST 84, 286
- EST 17,78
- mass spectra 54, 59, 66
- nucleic acid 82
- protein 82
- sequence homology 84
- sequence search 8
- sequest 8
deblocking aminopeptidase (DAP) 313
deblocking, proteins
- chemical
- - alcoholic deacetylation 315
- - N -terminal acetylserine/acetylthreonine
313,315
- - N-terminal pyroglutamic acid 315
- enzymatic 304
- - acylamino acid releasing enzyme 311-313
- - aminopeptidase 304-310
- - N-terminal pyroglutamic acid 310
- sample preparation 317
- sequential 319,317
deglycosilation, micro- 40
dermorphin 201
desalting 177
destaining 83
dextran 324
dichroism, circular 213,215, 216
Subject Index
differential scanning technique 65, 69, 77, 78
digestion 93
- buffer 40
- enzymatic 66, 354
- in -gel 45, 50, 81-85
- in-situ 21
- peptide 18,51,55
- protease 333
- protein 61, 177
- tryptic fragments 83, 180
dipolar contribution 103
disaccharide 343, 346, 347
- o-disaccharides 359, 361
disulfide 194, 196, 197
- bond, MALDI-TOF-MS 131
- bridges 247
- folding 190
- hydrophobic interactions 211,212
- native bonds 189
- scrambled 192
DITC 317
DNA
- binding properties 257
- nucleosomal 268
- sequencing 25,81, 175
- - cDNA 15,24
- - database 15
- - genomic 16
domains 247, 333
- C-terminal 293
- C-terminal-trans membrane 292
- catalytic 295-297
- CCP 337
- CH2 326
- extracellular 332
- functional 258
- heme binding 149, 153, 154
- hydrophobic 218
- saposin 252, 253
- single 189
Drosophila melanogaster
- antimicrobial peptides 158
- defense reaction 157 -172
- genome 170
- hemolymph 161-163, 168, 171
- immunity 158
- morphological level 158
- septic injury 158
- sequence 84
E
ECO RI 151
Edman degradation 24, 85, 86, 175, 185, 303,
313,317