- - epidermal growth factor
(EGF) 126
- - mouse nerve growth factor
(NGF) cDNA 117
- rRNA (see there) 55, 168
- in situ hybridzation 115 - 127,
128-137,139-152,154-171
digoxigenin 174
- - positive control 117
non-radioactive probe 52,
139-152, 154-171, 172-181,
182-195,196-203
- - oligo-DNA probe: T-T dimer
154-171
RNA probe 196-203
- - RNA synthesis kit 117
ss cDNA probe 147
- - with T -T dimerized ss cDNA
probe 140
RNase treatment 168, 180, 192
- cRNA, prehybridization
(RNase-free condition) 199
- digoxigenin 180, 192
- pretreatment 168
rough endoplasmatic reticulum
211-217
rRNA (ribosomal RNA) 55, 168
- 28S rRNA 61, 168, 175, 180, 187
[35s1-rUTP 120
S
SDS 135, 179
secretory granules 216,217
sectioning 119
- frozen section with digoxigenin
(Dig) 26
sense probe study 192
silane-coated glass slides 9
simultaneous detection 232
single copy hybridization 77
single-stranded breaks, DNA
(SSB) 3, 13, 19
size of probe 53
SKY (spectral karyotyping) 65
slide glass preparation 7,9,74, 119
- for cell specimen 77
- gelatin-coated glass slides 7
- in rapid protocol 89
- silane-coated glass slides 9
Subject Index
261
- washing the slide (see there) 76,
79, 89
small intestine 241
snapback 6
southwestern histochemistry 240,
242, 244 - 252
- analysis 244
- blot analysis 245
- fresh frozen sections 246
- oligo-DNAs 245
- practice 245
- T -T dimers 246
- transcriptional activity 240, 242
spectral karyotyping (SKY) 65
split phenomenon 82
ss (single-stranded) cDNA (see also
ds double stranded) 129 -153
- biotin-ll-dUTP 140
- digoxigenin-ll-dUTP 140
- oligonucleotide probe and ss
cDNA 150
- in situ hybridization 147
SSB (single-stranded breaks),
DNA 3,13,19
sse 106
staining reagents 71, 124
- fluorescent (see there) 71, 79
- methyl green/pyronin Y staining 165
- RNA 124
streptavidin
- gold/streptavidin colloidal gold
208, 209, 214, 215
- streptavidin-biotin-horseradish
peroxidase 208
stringent/stringency
- condition, digoxigenin 180, 189
- washing (see there) 166
synoviocytes 112
T
TdT (terminal deoxynucleotidyl
transferase) 8, 9, 23, 35
- reaction solution-digoxigenin
26
- TdT-mediated dUTP-biotin nick
end labeling 35
TEM (transmission electron microscopy) 31,32
(EGF) 126
- - mouse nerve growth factor
(NGF) cDNA 117
- rRNA (see there) 55, 168
- in situ hybridzation 115 - 127,
128-137,139-152,154-171
digoxigenin 174
- - positive control 117
non-radioactive probe 52,
139-152, 154-171, 172-181,
182-195,196-203
- - oligo-DNA probe: T-T dimer
154-171
RNA probe 196-203
- - RNA synthesis kit 117
ss cDNA probe 147
- - with T -T dimerized ss cDNA
probe 140
RNase treatment 168, 180, 192
- cRNA, prehybridization
(RNase-free condition) 199
- digoxigenin 180, 192
- pretreatment 168
rough endoplasmatic reticulum
211-217
rRNA (ribosomal RNA) 55, 168
- 28S rRNA 61, 168, 175, 180, 187
[35s1-rUTP 120
S
SDS 135, 179
secretory granules 216,217
sectioning 119
- frozen section with digoxigenin
(Dig) 26
sense probe study 192
silane-coated glass slides 9
simultaneous detection 232
single copy hybridization 77
single-stranded breaks, DNA
(SSB) 3, 13, 19
size of probe 53
SKY (spectral karyotyping) 65
slide glass preparation 7,9,74, 119
- for cell specimen 77
- gelatin-coated glass slides 7
- in rapid protocol 89
- silane-coated glass slides 9
Subject Index
261
- washing the slide (see there) 76,
79, 89
small intestine 241
snapback 6
southwestern histochemistry 240,
242, 244 - 252
- analysis 244
- blot analysis 245
- fresh frozen sections 246
- oligo-DNAs 245
- practice 245
- T -T dimers 246
- transcriptional activity 240, 242
spectral karyotyping (SKY) 65
split phenomenon 82
ss (single-stranded) cDNA (see also
ds double stranded) 129 -153
- biotin-ll-dUTP 140
- digoxigenin-ll-dUTP 140
- oligonucleotide probe and ss
cDNA 150
- in situ hybridization 147
SSB (single-stranded breaks),
DNA 3,13,19
sse 106
staining reagents 71, 124
- fluorescent (see there) 71, 79
- methyl green/pyronin Y staining 165
- RNA 124
streptavidin
- gold/streptavidin colloidal gold
208, 209, 214, 215
- streptavidin-biotin-horseradish
peroxidase 208
stringent/stringency
- condition, digoxigenin 180, 189
- washing (see there) 166
synoviocytes 112
T
TdT (terminal deoxynucleotidyl
transferase) 8, 9, 23, 35
- reaction solution-digoxigenin
26
- TdT-mediated dUTP-biotin nick
end labeling 35
TEM (transmission electron microscopy) 31,32