262
Subject Index
- ISELITEM technique (see there)
32,35,43
temperature, melting (Tm) 51,156,
157
template DNA preparation 196
terminal
- deoxynucleotidyl transferase
(see TdT) 8, 9, 23, 26
- dUTP nick end labeling
(see TUNEL) 8
- transferase-mediated dUTPbiotin nick end labeling
(see TUNEL) 19, 35
testis 9
thymine-thymine (see T-T) 51,
128-133,140,154-171,183
tissue preparation 55
Tm (melting temperature) 51,156,
157
TNEB 109
transcriptional activity 237 - 243
- general transcription factors
238
- regulatory factors 237, 238, 244
- - gene specific 238
- reverse transcripted cDNA 142
- in vitro transcription, RNA
probe 196
translocations 67, 81
transmission electron microscopy
(see TEM) 31, 32
trisomies 67
tritium labeled probes 102
Triton X-lOO 165
troubleshooting 84 - 88
- in CGH 98
- in codenaturation 91
- distored cell morphology 88
- high slide backround 87
- low signal specificity 87
T -T (thymine-thymine) 51,
128-133,140,154-170,183
- anti-T-T mouse IgG 131
- dimerization/dimer method 51,
12~ 12~ 133, 154-17~ 183
- - competition experiment 170
- - of DNA 155
- - immunohistochemical
detection 157
- - of oligo-DNA probes 160
- - pyrimidine ring of thymidine
154
- - in situ hybridization with T -T
dimer method 154
- - transcriptional activity 240
- - UV rays 154
- dot blot hybridization with T -T
dimerized DNA probes 158,162,
167
- HRP-Iabeled mouse anti-T-T
antibody 130
- localization in situ of specific
mRN A using T -T dimerized
oligo-DNA probes 164
- southwestern histochemistry
246
- ss cDNA probe 140
TTA repeats 157
tumor
- deletion of suppressor genes 67
- intra-tumoral heterogeneity 82
- melanoma cells 83
TUNEL
- of ischemia-reperfusion
kidneys 27, 28
- terminal dUTP nick end
labeling 8
- terminal transferase-mediated
dUTP-biotin nick end labeling
19,35
tyramine, biotinylated 60
U
uterus 59
UV
- irradiation 51
- radiation 7
- - UV rays 154
W
washing
- condition 189
- embryos 231
- hybridization and washing in
CGH 96
- posthybridization washing 200
- RNA 122
- the slide 76, 79, 89
Subject Index
- ISELITEM technique (see there)
32,35,43
temperature, melting (Tm) 51,156,
157
template DNA preparation 196
terminal
- deoxynucleotidyl transferase
(see TdT) 8, 9, 23, 26
- dUTP nick end labeling
(see TUNEL) 8
- transferase-mediated dUTPbiotin nick end labeling
(see TUNEL) 19, 35
testis 9
thymine-thymine (see T-T) 51,
128-133,140,154-171,183
tissue preparation 55
Tm (melting temperature) 51,156,
157
TNEB 109
transcriptional activity 237 - 243
- general transcription factors
238
- regulatory factors 237, 238, 244
- - gene specific 238
- reverse transcripted cDNA 142
- in vitro transcription, RNA
probe 196
translocations 67, 81
transmission electron microscopy
(see TEM) 31, 32
trisomies 67
tritium labeled probes 102
Triton X-lOO 165
troubleshooting 84 - 88
- in CGH 98
- in codenaturation 91
- distored cell morphology 88
- high slide backround 87
- low signal specificity 87
T -T (thymine-thymine) 51,
128-133,140,154-170,183
- anti-T-T mouse IgG 131
- dimerization/dimer method 51,
12~ 12~ 133, 154-17~ 183
- - competition experiment 170
- - of DNA 155
- - immunohistochemical
detection 157
- - of oligo-DNA probes 160
- - pyrimidine ring of thymidine
154
- - in situ hybridization with T -T
dimer method 154
- - transcriptional activity 240
- - UV rays 154
- dot blot hybridization with T -T
dimerized DNA probes 158,162,
167
- HRP-Iabeled mouse anti-T-T
antibody 130
- localization in situ of specific
mRN A using T -T dimerized
oligo-DNA probes 164
- southwestern histochemistry
246
- ss cDNA probe 140
TTA repeats 157
tumor
- deletion of suppressor genes 67
- intra-tumoral heterogeneity 82
- melanoma cells 83
TUNEL
- of ischemia-reperfusion
kidneys 27, 28
- terminal dUTP nick end
labeling 8
- terminal transferase-mediated
dUTP-biotin nick end labeling
19,35
tyramine, biotinylated 60
U
uterus 59
UV
- irradiation 51
- radiation 7
- - UV rays 154
W
washing
- condition 189
- embryos 231
- hybridization and washing in
CGH 96
- posthybridization washing 200
- RNA 122
- the slide 76, 79, 89