260
Subject Index
p
painting, whole painting probe 84
paraffin
- blocks 104
- embedding 119
parafilm® 34
paraformaldehyde (PFA) 104,118,
172, 185,225,247,248
- 4 % 104, 185,247,248
- fixative 118
pBR-322 240
PCR
- asymmetric 140 -148
- - single step 145, 148
- - two-step 141, 148
- first 141, 143
- second 141, 145
- symmetric, two-step 139
perfuse 119
Philadelphia chromosome 67, 84
pituitary gland 238
PK digestion 17
PN buffer 72
PNM buffer 70, 72
polyvinyl alcohol 227
POMC (proopiomelanocortin) 240
positive control, contents in ISH
kit 117
posthybridization washing 200
prehybridzation 108, 173
- cRNA 199
- digoxigenin 173
- RNase-free condition 199
- solution 163
pretreatment 56
- embryois 229
- RNA 122
- with RNase 168
primer design 142
PRL 211
probe concentration 132, 248
- control experiments 249
- NaCl 249
proopiomelanocortin (POMC) 240
propidium iodide (PI) or DAPI,
slide washing 76
protease treatment 56
protein A colloidal gold 208, 210,
216
proteinase K 6, 156, 165, 178,225
- treatment 24
proteolysis 57
pUC 18 plasmid 133
pyrimidine ring of thymidine 154
pyronin Y staining/methyl green
165, 186
R
radiation, UV radiation 7
radioactive (RI) probe 115
DNA probe 100-114
- non-radioactive (see there) 14,
50, 52
- RI-labeled riboprobes 120
radioisotopes 50, 101
radio nuclides used in situ
hybridization 102
rapid procedure 88
reaction time (T) 197
recommended size of probe 128
regulatory element 250
repetitive probes 75, 76
- chromosome-specific repetitive
sequences 75, 78
reporter deposition, catalyzed 60
resin
- Epon resin 207, 209
- LR white resin 207,209,210,216
responsive element 238
restriction endonuclease 129, 132
reverse transcripted cDNA 142
rheumatoid arthritis 112
riboprobes, RI-labeled 120
RNA
- concentration measurement
202
- cRNA (see there) 197, 198
- detection of specific DNA and
RNA sequences 49-62
- digoxigenin-labeled RNA
probes 222
- hybridization of 55
- mRNA (see there) 59, 111, 151,
164,204,219,222-234
- polymerase 120
- probe synthesis 117
- - alkaline hydrolysis 117, 121
Subject Index
p
painting, whole painting probe 84
paraffin
- blocks 104
- embedding 119
parafilm® 34
paraformaldehyde (PFA) 104,118,
172, 185,225,247,248
- 4 % 104, 185,247,248
- fixative 118
pBR-322 240
PCR
- asymmetric 140 -148
- - single step 145, 148
- - two-step 141, 148
- first 141, 143
- second 141, 145
- symmetric, two-step 139
perfuse 119
Philadelphia chromosome 67, 84
pituitary gland 238
PK digestion 17
PN buffer 72
PNM buffer 70, 72
polyvinyl alcohol 227
POMC (proopiomelanocortin) 240
positive control, contents in ISH
kit 117
posthybridization washing 200
prehybridzation 108, 173
- cRNA 199
- digoxigenin 173
- RNase-free condition 199
- solution 163
pretreatment 56
- embryois 229
- RNA 122
- with RNase 168
primer design 142
PRL 211
probe concentration 132, 248
- control experiments 249
- NaCl 249
proopiomelanocortin (POMC) 240
propidium iodide (PI) or DAPI,
slide washing 76
protease treatment 56
protein A colloidal gold 208, 210,
216
proteinase K 6, 156, 165, 178,225
- treatment 24
proteolysis 57
pUC 18 plasmid 133
pyrimidine ring of thymidine 154
pyronin Y staining/methyl green
165, 186
R
radiation, UV radiation 7
radioactive (RI) probe 115
DNA probe 100-114
- non-radioactive (see there) 14,
50, 52
- RI-labeled riboprobes 120
radioisotopes 50, 101
radio nuclides used in situ
hybridization 102
rapid procedure 88
reaction time (T) 197
recommended size of probe 128
regulatory element 250
repetitive probes 75, 76
- chromosome-specific repetitive
sequences 75, 78
reporter deposition, catalyzed 60
resin
- Epon resin 207, 209
- LR white resin 207,209,210,216
responsive element 238
restriction endonuclease 129, 132
reverse transcripted cDNA 142
rheumatoid arthritis 112
riboprobes, RI-labeled 120
RNA
- concentration measurement
202
- cRNA (see there) 197, 198
- detection of specific DNA and
RNA sequences 49-62
- digoxigenin-labeled RNA
probes 222
- hybridization of 55
- mRNA (see there) 59, 111, 151,
164,204,219,222-234
- polymerase 120
- probe synthesis 117
- - alkaline hydrolysis 117, 121