M
M13 phage vector 139
master mix 73, 75, 78
melanoma cells 83
metaphase 65
methallothionein (MT) 169
methyl green 45
- methyl green/pyronin Y
staining 165, 186
M-FISH 65
micro deletions 67
microscopy
- confocal laser microscopy
(CLM) 44
electron microscopy
- postembedding 206,218
- pre embedding 205,218
- in situ hybridization and
combination with immunohistochemistry 204 - 220
- transmission (see TEM) 31,32
RNA 124
- in situ nick end-labeling 19 - 30,
31-46
MMP-1 mRNA 112, 113
MMP-3 mRNA 113
monosomies 67
mRNA 59, Ill, 164,222-234
- estrogen receptor (ER) mRNA
59
- IL-1~ mRNA 151
- IL-4 193
- IL-lO mRNA 180
- localization in situ of specific
mRN A using T -T dimerized
oligo-DNA probes 164
- MMP-1 mRNA 112,113
- type II collagen Ill, 112
- ultrastructural in situ hibridization for mRNA 204,2l9
- whole mount in situ hybridization for mRNA 222 - 234
N
NaCI concentration 249
necrosis 3, 10, 17
- ISELITEM technique, necrotic
cell labeled with 43
Subject Index
259
NGF (nerve growth factor) 117
nick
- CGH, nick translation of DNA
samples for comparative
genomic hybridization 95
dUTP nick end labeling
(see TUNEL) 8
TdT -mediated dUTP-biotin nick
end labeling 35
transferase-mediated dUTPbiotin nick end labeling
(see TUNEL) 19,35
- translation 4, 5, 13, 103
- - in situ nick translation
(see ISNT) 4, 13 - 17
nickel grids 33
NIH image program 37,41
non-radioactive probe 50
- in situ hybridization 52,
139-152,154-171,172-181,
182-195,196-203
- ISNT, principle of 14
- labels 50
- oligo-DNA probe: digoxigenin
172-181
- RNA probe 196-203
NP3 cell 42
nucleoid sedimentation technique
3
o
oncology by FISH 67
oligo-deoxynucleotide ( oligo-DNA)
probe 51, 157, 160
- design 157
- non-radioactive probe for RNA:
oligo-DNA probe: digoxigenin
172-181,182-194
- southwestern histochemistry
245
- T -T dimerization of 160
- TTA repeats 157
oligo-nucleotide probes
- biotinylated synthesized 204
- and ss cDNA 150
oncogenes, amplifications of 67
oxidant treatment 38