258
Subject Index
- digoxigenin 181
- . NIH image program 37, 41
immunogold particles 32, 38
immunohistochemistry/immunohistochemical detection 49, 161,
176
- digoxigenin 173, 176
- electron microscopy in situ
hybridization and combination
with immunohistochemistry
204-220
immunoperoxidase method,
three-layer 190
- with anti-Dig antibody 190
- with HRP-conjugated
antibodies 190
in situ
- hybridization 49, 51, 52, 58,
115 -127, 128 -138, 139 -153,
154-171,204-220,222-234
- direct method 129, 134,
- for DNA: fluorescent probe
65-98
- - electron microscopy in situ
hybridization and combination with immunohistochemistry 204 - 220
- - fluorescent in situ hybridization (see FISH) 65 - 90
- - indirect method 136
- - non-radioactive probe
52,
139-152, 154-171, 172-181,
182-195,196-203
for RNA (see there) 115 - 127,
128-138, 139-153, 154-171,
204
- - ss cDNA probe 147
- - sensitivity of 58
- - T-T dimer 154-171
- - ultrastructural in situ hibridization for mRNA 204
- - whole mount in situ hybridization for mRNA 222-234
- localization in situ of specific
mRNA using T -T dimerized
oligo-DNA probes 164
- nick end-labeling 19 - 30,31- 46
- - electron microscopical 31-46
- - light microscopical 19 - 30
- nick translation (see ISNT) 4,
13-17
in vitro transcription, RNA probe
196
inhibitor of DNA synthesis 42
interleukin (see IL)
interphase 65
- nuclei 81
inversions 81
irradiation 51, 177
- digoxigenin 177
- UV irradiation 51
ischemia-reperfusion kidneys,
TUNEL 27,28
ISEL 31
- equilibration buffer for the ISEL
technique 34
ISELITEM technique 32, 35
- necrotic cell labeled with 43
ISH 115
- kit 115
- method 115
- positive control, contents in ISH
kit 117
ISNT (in situ nick translation) 4,
13-17
- principle of non-radioactive
ISNT 14
K
karyotyping, spectral (SKY) 65
L
labeling-densitiy for DNA strand
breaks 37
labeling-protocols 53
lambda phage DNA 133
laser, confocal laser microscopy
(eLM) 44
locking forceps 35
locus-specific probes, dual-color
FISH using a combination of
centromeric and locus-specific
probes 86
Lowicryl K4M 218
LR white resin 207, 209, 210, 216
lymphocytes, human peripheral
blood lymphocytes (PBL) 4,6, 7
Subject Index
- digoxigenin 181
- . NIH image program 37, 41
immunogold particles 32, 38
immunohistochemistry/immunohistochemical detection 49, 161,
176
- digoxigenin 173, 176
- electron microscopy in situ
hybridization and combination
with immunohistochemistry
204-220
immunoperoxidase method,
three-layer 190
- with anti-Dig antibody 190
- with HRP-conjugated
antibodies 190
in situ
- hybridization 49, 51, 52, 58,
115 -127, 128 -138, 139 -153,
154-171,204-220,222-234
- direct method 129, 134,
- for DNA: fluorescent probe
65-98
- - electron microscopy in situ
hybridization and combination with immunohistochemistry 204 - 220
- - fluorescent in situ hybridization (see FISH) 65 - 90
- - indirect method 136
- - non-radioactive probe
52,
139-152, 154-171, 172-181,
182-195,196-203
for RNA (see there) 115 - 127,
128-138, 139-153, 154-171,
204
- - ss cDNA probe 147
- - sensitivity of 58
- - T-T dimer 154-171
- - ultrastructural in situ hibridization for mRNA 204
- - whole mount in situ hybridization for mRNA 222-234
- localization in situ of specific
mRNA using T -T dimerized
oligo-DNA probes 164
- nick end-labeling 19 - 30,31- 46
- - electron microscopical 31-46
- - light microscopical 19 - 30
- nick translation (see ISNT) 4,
13-17
in vitro transcription, RNA probe
196
inhibitor of DNA synthesis 42
interleukin (see IL)
interphase 65
- nuclei 81
inversions 81
irradiation 51, 177
- digoxigenin 177
- UV irradiation 51
ischemia-reperfusion kidneys,
TUNEL 27,28
ISEL 31
- equilibration buffer for the ISEL
technique 34
ISELITEM technique 32, 35
- necrotic cell labeled with 43
ISH 115
- kit 115
- method 115
- positive control, contents in ISH
kit 117
ISNT (in situ nick translation) 4,
13-17
- principle of non-radioactive
ISNT 14
K
karyotyping, spectral (SKY) 65
L
labeling-densitiy for DNA strand
breaks 37
labeling-protocols 53
lambda phage DNA 133
laser, confocal laser microscopy
(eLM) 44
locking forceps 35
locus-specific probes, dual-color
FISH using a combination of
centromeric and locus-specific
probes 86
Lowicryl K4M 218
LR white resin 207, 209, 210, 216
lymphocytes, human peripheral
blood lymphocytes (PBL) 4,6, 7