- in situ hybridization (see FISH)
65-90
- - for DNA: fluorescent probe
65-98
- staining reagents 71
- - biotinized probes 79
- - digoxigenin labeled probes 81
fluorochrome-directly-Iabeled
DNA probe 65-90
forceps 35
- anticapillary 35
- locking 35
formam ide 109, 185
fresh frozen sections 246
G
gelatin-coated glass slides 7
gene specific transcription
regulatory factors 238
genetic diagnosis by FISH 66
- postnatal 66
- preimplantation 66
- prenatal 66
genome/genomic
- DNA 78
- loci of probes 81
GH 211
glass slides, silane-coated 9
glucocorticoid
- receptor (GR) 240
- response element (GRE) 242
gold
- immunogold particles 32, 38
- colloidal gold 37
- protein A colloidal gold 208,
210,216
- streptavidin gold 208, 209, 214,
215
- - colloidal gold 208
H
hapten 183
heparin 135, 179
HER-2 85
HRP (horseradish peroxides), HRPlabeled 23,52,130, 131, 173
- goat anti-mouse IgG 131
- mouse anti-T -T antibody 130
- sheep anti-digoxigenin
antibody 173
Subject Index
257
- streptavidin-biotin-horseradish
peroxidase 208
- three-layer immunoperoxidase
method 190
hybridization 78
- comparative genomic hybridization (see CGH) 66,68,85,92 - 98
dot blot 54, 154, 158, 162, 167,
173, 176
embryos 229
filter hybridization 34, 149
mix preparation 75, 78, 88, 109,
159,230
in digoxigenin 173
- in embryos 230
in rapid protocol 88
- in situ hybridization with T -T
dimerized probes 159
posthybridization (see there)200
prehybridzation (see there) 108,
163, 174, 199
in rapid protocol 89
radio nuclides used in situ
hybridization 102
RNA 55,122
RNase-free condition 199
single copy hybridization 77
in situ (see there) 49, 51, 52, 58,
115 -127, 128 -138, 139 -153,
154-171,204,222-234
- solution 163
- T-T dimer 166
hydroxyapatite column 149
hyperthermia 42
I
IgG
- anti-T-T mouse 131
- HRP-Iabeled goat anti-mouse
IgG 131
IL (interleukin)
- IL-1 113
- IL-1~ mRNA 151
- IL-lO mRNA 180
illumination, dark-field 125
image analysis 32, 40