256
Subject Index
- digoxigenin 173, 176
- with T -T dimerized DNA probes
158, 162, 167
ds (double-stranded;
(see also ss single stranded)
- breaks (DSB) 3, 45
- ds cDNA probe 128, 129
- - digestion of 129
DSB (double-stranded breaks) 3,
45
dTTP 101
- 3H-labeled dTTP cDNA probe
101
- 35S-labeled dTTP cDNA probe
101
dual-color FISH (see also FISH) 67,
68, 83
- using a combination of centromeric and locus-specific probes 86
duplications 66
dUTP
- biotin-11-dUTP 5
- digoxigenin-11-dUTP
(see there) 5, 19, 23, 26
- fluorescent conjugated dUTP 19
- TdT-mediated dUTP-biotin nick
end labeling 35
- terminal dUTP nick end labeling
(TUNEL) 8
- terminal transferase-mediated
dUTP-biotin nick end labeling
19,35
E
EGF (epidermal growth factor) 126
- RNA 126
EGFR (epidermal growth factor
receptor) gene 244
- transcription factor, EGFR
specific (see ETF) 250
electron microscopy (see also
microscopy)
- in situ hybridization and combination with immunohistochemistry 204 - 220
- - postembedding 206,218
- - preembedding 205, 218
- transmission (see TEM) 31,32
embryos 222 - 234
- embryo powder 226
- hybridization 226
- pretreatment 229
emulsion, RNA 123
endogenous peroxidase 178
endonuclease
- restriction 129, 132
- SI endonuclease 146
endoplasmatic reticulum, rough
211-217
epidermal growth factor (see EGF)
126
Epon resin 207, 209
esophageal cancer 169
estrogen receptor (ER) mRNA 59
etching 219
ETF (EGFR specific transcription
factor) 250
- localization in frozen sections
250
ethanol precipitation 176
exposure time 110
F
fIlter hybridization 34, 149
FISH (fluorescent in situ hybridization) 65 - 90
- chemically modified DNA
probes 76
- dual-color FISH (see there) 67,
68,83,86
- fluorochrome-directly-labeled
DNA probes 65-90
- genetic diagnosis by FISH
(see there) 66
- methanol-acetic acid (3: 1)
fixation 69
- multi-target FISH (M-FISH) 65
- oncology by FISH 67
- PNM buffer 70, 72
- propidium iodide (PI) 70
fixation 56, 229, 248
fluorescent
- amplifications of biotinized
probe fluorescence 80
- autofluorescent 45
- conjugated dUTP 19
- fluorescent dye 60
Subject Index
- digoxigenin 173, 176
- with T -T dimerized DNA probes
158, 162, 167
ds (double-stranded;
(see also ss single stranded)
- breaks (DSB) 3, 45
- ds cDNA probe 128, 129
- - digestion of 129
DSB (double-stranded breaks) 3,
45
dTTP 101
- 3H-labeled dTTP cDNA probe
101
- 35S-labeled dTTP cDNA probe
101
dual-color FISH (see also FISH) 67,
68, 83
- using a combination of centromeric and locus-specific probes 86
duplications 66
dUTP
- biotin-11-dUTP 5
- digoxigenin-11-dUTP
(see there) 5, 19, 23, 26
- fluorescent conjugated dUTP 19
- TdT-mediated dUTP-biotin nick
end labeling 35
- terminal dUTP nick end labeling
(TUNEL) 8
- terminal transferase-mediated
dUTP-biotin nick end labeling
19,35
E
EGF (epidermal growth factor) 126
- RNA 126
EGFR (epidermal growth factor
receptor) gene 244
- transcription factor, EGFR
specific (see ETF) 250
electron microscopy (see also
microscopy)
- in situ hybridization and combination with immunohistochemistry 204 - 220
- - postembedding 206,218
- - preembedding 205, 218
- transmission (see TEM) 31,32
embryos 222 - 234
- embryo powder 226
- hybridization 226
- pretreatment 229
emulsion, RNA 123
endogenous peroxidase 178
endonuclease
- restriction 129, 132
- SI endonuclease 146
endoplasmatic reticulum, rough
211-217
epidermal growth factor (see EGF)
126
Epon resin 207, 209
esophageal cancer 169
estrogen receptor (ER) mRNA 59
etching 219
ETF (EGFR specific transcription
factor) 250
- localization in frozen sections
250
ethanol precipitation 176
exposure time 110
F
fIlter hybridization 34, 149
FISH (fluorescent in situ hybridization) 65 - 90
- chemically modified DNA
probes 76
- dual-color FISH (see there) 67,
68,83,86
- fluorochrome-directly-labeled
DNA probes 65-90
- genetic diagnosis by FISH
(see there) 66
- methanol-acetic acid (3: 1)
fixation 69
- multi-target FISH (M-FISH) 65
- oncology by FISH 67
- PNM buffer 70, 72
- propidium iodide (PI) 70
fixation 56, 229, 248
fluorescent
- amplifications of biotinized
probe fluorescence 80
- autofluorescent 45
- conjugated dUTP 19
- fluorescent dye 60