Denhardt 105, 106
- Denhardt's mixture 105
deoxynucleotidyl transferase,
terminal (see TdT) 8, 9, 23, 26
deparaffinization, RNA 122
deproteinization 188
destaining, whole mount in situ
hybridization for mRNA 232
development 110
- biology, develpmental 112
- RNA 124
dextran sulfate 109
diethylpyrocarbonate 184
digoxigenin (Dig) 51, 183
- accessibility of probe 188
- anti-digoxigenin
- - antibody conjugated with
alkaline phosphatase 201
- - anti-Dig-AP 226
- - sheep anti-digoxigenin
antibody 173
- - three-layer immunoperoxidase method 190
- competitive study 192
- control experiments 170
- delipidation 179
- digoxigenin-ll-dUTP 5,19,23
- digoxigenin-Iabeled probes 81
- deproteinization 188
- frozen section with 26
- homology -176
- HRP-Iabeled sheep antidigoxigenin antibody 173
- non-radioactive probe for RNA:
oligo-DNA probe 172-181,
182-194
- - hybridization mixture 174
- - prehybridization mixture 174
- - preparation of tissues 172
- - tailing kit 173
- RNase treatment 192
- RNA probes, digoxigeninlabeled 222
ss cDNA probe 140
- sense probe study 192
- specifity of the signal 192
- TdT reaction solutiondigoxigenin 26
- transcriptional activity 240
Subject Index
255
DNA
- cDNA probe (see there) 101,128,
129
- - ss cDNA probe 139-153
- CGH, nick translation of DNA
samples for comparative
genomic hybridization 95
- detection of specific DNA
and RNA sequences 49-62
- FISH using
- - chemically modified DNA
probes 76
- - fluorochrome-directly-Iabeled
DNA probes 73
- fragmentation 31
- genomic DNA 78
- inhibitor of DNA synthesis 42
- lambda phage DNA 133
- localization in situ of specific
mRN A using T -T dimerized
oligo-DNA probes 164
- mouse nerve growth factor (NGF)
cDNA 117
- oligodeoxynuclotide
(oligo-DNA) probe 51
- polymerase I 5, 15
- preparation of template DNA
196
- radioactive DNA probe
100-114
- in situ hybridization for DNA:
fluorescent probe 65 - 98
- southwestern histochemistry,
oligo-DNAs 245
- strand breaks 3 - 10
- - DSB (double-stranded
breaks) 3, 45
- - labeling densitiy for DNA
strand breaks 37
- - SSB (single-stranded breaks)
3, 13, 19
- T-T (thymine-thymine)
dimerized DNA 154, 155
- remove interfering proteins
156
DNase I 129
dot blot hybridization 52, 154, 158,
162,167,173,176
- colorimetric 167
- Denhardt's mixture 105
deoxynucleotidyl transferase,
terminal (see TdT) 8, 9, 23, 26
deparaffinization, RNA 122
deproteinization 188
destaining, whole mount in situ
hybridization for mRNA 232
development 110
- biology, develpmental 112
- RNA 124
dextran sulfate 109
diethylpyrocarbonate 184
digoxigenin (Dig) 51, 183
- accessibility of probe 188
- anti-digoxigenin
- - antibody conjugated with
alkaline phosphatase 201
- - anti-Dig-AP 226
- - sheep anti-digoxigenin
antibody 173
- - three-layer immunoperoxidase method 190
- competitive study 192
- control experiments 170
- delipidation 179
- digoxigenin-ll-dUTP 5,19,23
- digoxigenin-Iabeled probes 81
- deproteinization 188
- frozen section with 26
- homology -176
- HRP-Iabeled sheep antidigoxigenin antibody 173
- non-radioactive probe for RNA:
oligo-DNA probe 172-181,
182-194
- - hybridization mixture 174
- - prehybridization mixture 174
- - preparation of tissues 172
- - tailing kit 173
- RNase treatment 192
- RNA probes, digoxigeninlabeled 222
ss cDNA probe 140
- sense probe study 192
- specifity of the signal 192
- TdT reaction solutiondigoxigenin 26
- transcriptional activity 240
Subject Index
255
DNA
- cDNA probe (see there) 101,128,
129
- - ss cDNA probe 139-153
- CGH, nick translation of DNA
samples for comparative
genomic hybridization 95
- detection of specific DNA
and RNA sequences 49-62
- FISH using
- - chemically modified DNA
probes 76
- - fluorochrome-directly-Iabeled
DNA probes 73
- fragmentation 31
- genomic DNA 78
- inhibitor of DNA synthesis 42
- lambda phage DNA 133
- localization in situ of specific
mRN A using T -T dimerized
oligo-DNA probes 164
- mouse nerve growth factor (NGF)
cDNA 117
- oligodeoxynuclotide
(oligo-DNA) probe 51
- polymerase I 5, 15
- preparation of template DNA
196
- radioactive DNA probe
100-114
- in situ hybridization for DNA:
fluorescent probe 65 - 98
- southwestern histochemistry,
oligo-DNAs 245
- strand breaks 3 - 10
- - DSB (double-stranded
breaks) 3, 45
- - labeling densitiy for DNA
strand breaks 37
- - SSB (single-stranded breaks)
3, 13, 19
- T-T (thymine-thymine)
dimerized DNA 154, 155
- remove interfering proteins
156
DNase I 129
dot blot hybridization 52, 154, 158,
162,167,173,176
- colorimetric 167