254
Subject Index
catalyzed
- reporter deposition 60
- signal amplification (SCA) 61
cDNA probe 101, 128, 129
- double-stranded cDNA probe
(see ds cDNA) 128, 129
- 3H-Iabeled dTTP 101
- reverse transcripted cDNA 142
- 35S-labeled dTTP 101
- ss cDNA probe (see ss cDNA)
139 -153
- template cDNA preparation 142
centromeric
- dual-color FISH using a
combination of centromeric
and locus-specific probes 86
- probes 82, 83
- regions 81
CGH (comparative genomic
hybridization) 66, 68, 85, 92 - 98
- hybridization and washing in
CGH 96
- nick translation of DNA samples
for comparative genomic
hybridization 95
- troubleshooting in CGH 98
chemically modified DNA probe
65, 76
chemiluminescence 62
chorionic villi sampling (CVS) 66
chromatin condensation 43
chromogen solution 173
chromosome/chromsomal
- enumeration, chromosome 82
- numerical aberrations,
chromosomal 67, 81
- Philadelphia chromosome 67,
84
- probes
- - chromosome-specific 65
- - whole chromosomal probes
81
- repetitive sequences,
chromosome-specific 75, 78
- segments, whole chromosomal
81
- structural abnormalities 81
- tandem repeating in centromeric
regions 81
CLM (confocal laser microscopy)
44
codenaturation 90
- preparation of a slide for 90
- troubleshooting 91
collagen, type II Ill, 112
colorimetric dot blot
hybridization 167
color reaction, whole mount in situ
hybridization for mRNA 232
colorectal cancers 17
comparative genomic hybridization
(see CGH) 66, 68, 85, 92 - 98
competition experiment!
competitive study 170, 192
- T-T dimer 170
complementary bases 51
concentration of probe 132, 248
confocal laser microscopy (CLM)
44
control experiments 57 - 59, 180,
249
copy number changes 68
cord-centesis 67
CRE (cAMP response element) 242
- CRE proteins 249
cRNA 197, 198
- purification 197
- quantification 198
CVS (chorionic villi sampling) 66
cyclic adenosine 3',5' -monophosphate (see cAMP) 242
D
DAPI or propidium iodide (PI),
slide washing 76
dark-field illumination 125
decalcification 175, 176
- tissues, decalcified 176
- various decalcification
protocols 175
dehydration 229
deionized formamide 166
deletion 66, 81, 83
- micro deletions 67
- of tumor suppressor genes 67
delipidation 179
denaturation solution 71, 73
Subject Index
catalyzed
- reporter deposition 60
- signal amplification (SCA) 61
cDNA probe 101, 128, 129
- double-stranded cDNA probe
(see ds cDNA) 128, 129
- 3H-Iabeled dTTP 101
- reverse transcripted cDNA 142
- 35S-labeled dTTP 101
- ss cDNA probe (see ss cDNA)
139 -153
- template cDNA preparation 142
centromeric
- dual-color FISH using a
combination of centromeric
and locus-specific probes 86
- probes 82, 83
- regions 81
CGH (comparative genomic
hybridization) 66, 68, 85, 92 - 98
- hybridization and washing in
CGH 96
- nick translation of DNA samples
for comparative genomic
hybridization 95
- troubleshooting in CGH 98
chemically modified DNA probe
65, 76
chemiluminescence 62
chorionic villi sampling (CVS) 66
chromatin condensation 43
chromogen solution 173
chromosome/chromsomal
- enumeration, chromosome 82
- numerical aberrations,
chromosomal 67, 81
- Philadelphia chromosome 67,
84
- probes
- - chromosome-specific 65
- - whole chromosomal probes
81
- repetitive sequences,
chromosome-specific 75, 78
- segments, whole chromosomal
81
- structural abnormalities 81
- tandem repeating in centromeric
regions 81
CLM (confocal laser microscopy)
44
codenaturation 90
- preparation of a slide for 90
- troubleshooting 91
collagen, type II Ill, 112
colorimetric dot blot
hybridization 167
color reaction, whole mount in situ
hybridization for mRNA 232
colorectal cancers 17
comparative genomic hybridization
(see CGH) 66, 68, 85, 92 - 98
competition experiment!
competitive study 170, 192
- T-T dimer 170
complementary bases 51
concentration of probe 132, 248
confocal laser microscopy (CLM)
44
control experiments 57 - 59, 180,
249
copy number changes 68
cord-centesis 67
CRE (cAMP response element) 242
- CRE proteins 249
cRNA 197, 198
- purification 197
- quantification 198
CVS (chorionic villi sampling) 66
cyclic adenosine 3',5' -monophosphate (see cAMP) 242
D
DAPI or propidium iodide (PI),
slide washing 76
dark-field illumination 125
decalcification 175, 176
- tissues, decalcified 176
- various decalcification
protocols 175
dehydration 229
deionized formamide 166
deletion 66, 81, 83
- micro deletions 67
- of tumor suppressor genes 67
delipidation 179
denaturation solution 71, 73