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body and 20 nm protein A colloidal gold identifies rat GH protein on the secretory granules (Fig. 10). Colloidal gold signals for
rat GH protein are distributed mainly on the secretory granules,
and are also identified in the cisternae of the rough endoplasmic
reticulum (Fig. 10). The immunoreactivity retrieved after etching
process with 10% H20 2 is similar and comparable to that with 4%
sodium periodate. Immunohistochemical control experiment
combined with preembedding electron microscopic in situ hybridization method reveals no reactions of protein A colloidal
gold particles on the secretory granules (Fig. 11).
Combined immunohistochemistry and preembedding in situ hybrid i- .
zation at an electron microscopic level using LR White resin
Using LR White resin for tissue embedment, rat GH mRNA is
also localized on the polysomes of the entire rough endoplasmic
Fig. 11. Immunohistochemical control experiments at the ultrastructural level.
No positive reactions of protein A colloidal gold particles are observed on the
. secretory granules, whereas positive signals for rat GH mRNA are identified as
osmium black on the polysomes of the rough endoplasmic reticulum.
(bar=200nm)