15 Electron Microscopic in Situ Hybridization and its Combination
217
Fig. 12. Using LR White resin for tissue embedment, rat GH mRNA is also localized on the polysomes of the entire rough endoplasmic reticulum. Subsequent immunohistochemical staining identifies rat GH protein both on the secretory granules (arrows) and in the cisternae of the rough endoplasmic reticulum (arrow heads). (bar=200nm)
reticulum (Fig. 12). Subsequent immunohistochemical staining
identifies rat GH protein both on the secretory granules and in
the cisternae of the rough endoplasmic reticulum (Fig. 12).
Troubleshooting
• Prolonged fixation of the tissues will decrease the messages.
• Excessive treatment with proteinase K will destroy the morphology.
• Excessive etching process will shade off the reaction products
(osmium black) of mRNA.
217
Fig. 12. Using LR White resin for tissue embedment, rat GH mRNA is also localized on the polysomes of the entire rough endoplasmic reticulum. Subsequent immunohistochemical staining identifies rat GH protein both on the secretory granules (arrows) and in the cisternae of the rough endoplasmic reticulum (arrow heads). (bar=200nm)
reticulum (Fig. 12). Subsequent immunohistochemical staining
identifies rat GH protein both on the secretory granules and in
the cisternae of the rough endoplasmic reticulum (Fig. 12).
Troubleshooting
• Prolonged fixation of the tissues will decrease the messages.
• Excessive treatment with proteinase K will destroy the morphology.
• Excessive etching process will shade off the reaction products
(osmium black) of mRNA.