Changes in Metabolite Concentrations in Ischemic
Kidneys of Rabbits and in Transplanted Kidneys of Dogs
H. R. SCHOEN, and R. E. Voss
With 3 Figures
Abstract
The behavior of high-energy phosphates and of lactate and pyruvate was
investigated in 212 kidneys of rabbits at 24° following a period of ischemia ranging
from 15 sec to 10 hrs. Within the first 5 min the A TP content decreased by 70 %
and the AMP increased by 130%; lactate increased more slowly. The subsequent
breakdown of AMP was considerably protracted and persisted for 10 hrs. This
behaviour was the same in 12 transplanted canine kidneys. After only 15 min of
revascularization the resynthesis of A TP occurred in the transplanted organ. The
survival time and the functional duration of the transplants were determined in
22 dogs.
In order to perform renal transplantation, the renal blood flow has to
be interrupted totally. During this period of ischemia, the energy for the
structure-maintaining metabolism is obtained by anaerobic glycolysis only.
Therefore, the amounts of reserve of glucose, glycogen and high-energy
phosphates are decisive for the duration of the revival time, defined as the
maximal duration of ischemia which still permits the restoration of a verifiable function. Under normothermic conditions this amounts for the kidney to 150 min. In contrast to the myocardium, the reserves of glycogen
and phosphocreatin in the kidney are small. Freely available energy for the
endergonic reactions is made available by the breakdown of ATP mainly.
1. Investigations with Rabbit Kidneys
Method: After extirpation the kidneys were stored at 24° in humid containers. The metabolite contents were determined at fixed intervals by
enzyme-chemical and -optical methods. Control values were gained after
immediate in-situ freezing [5] using frozen crushing forceps.
Results: Within 5 min. of ischemia a rapid decrease of A TP, a less
pronounced decrease of ADP and a considerable accumulation of lowenergy AMP occurred. In contrast to myocardium and brain, glycolysis
Kidneys of Rabbits and in Transplanted Kidneys of Dogs
H. R. SCHOEN, and R. E. Voss
With 3 Figures
Abstract
The behavior of high-energy phosphates and of lactate and pyruvate was
investigated in 212 kidneys of rabbits at 24° following a period of ischemia ranging
from 15 sec to 10 hrs. Within the first 5 min the A TP content decreased by 70 %
and the AMP increased by 130%; lactate increased more slowly. The subsequent
breakdown of AMP was considerably protracted and persisted for 10 hrs. This
behaviour was the same in 12 transplanted canine kidneys. After only 15 min of
revascularization the resynthesis of A TP occurred in the transplanted organ. The
survival time and the functional duration of the transplants were determined in
22 dogs.
In order to perform renal transplantation, the renal blood flow has to
be interrupted totally. During this period of ischemia, the energy for the
structure-maintaining metabolism is obtained by anaerobic glycolysis only.
Therefore, the amounts of reserve of glucose, glycogen and high-energy
phosphates are decisive for the duration of the revival time, defined as the
maximal duration of ischemia which still permits the restoration of a verifiable function. Under normothermic conditions this amounts for the kidney to 150 min. In contrast to the myocardium, the reserves of glycogen
and phosphocreatin in the kidney are small. Freely available energy for the
endergonic reactions is made available by the breakdown of ATP mainly.
1. Investigations with Rabbit Kidneys
Method: After extirpation the kidneys were stored at 24° in humid containers. The metabolite contents were determined at fixed intervals by
enzyme-chemical and -optical methods. Control values were gained after
immediate in-situ freezing [5] using frozen crushing forceps.
Results: Within 5 min. of ischemia a rapid decrease of A TP, a less
pronounced decrease of ADP and a considerable accumulation of lowenergy AMP occurred. In contrast to myocardium and brain, glycolysis