Fig. 3. a) Control sedimentation profile of uri dine-labelled RNA (30 min pulse)
in a 5-25% sucrose-gradient (e---·e OD260 ; e-e counts per min).
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40
Fraction
60
80
1000
c::
E
a;
Q.
V)
C
:::J
0
U
500
b) Sedimentation profile of uri dine-labelled RNA (30 min pulse) in a 5-25 %
sucrose gradient 5 hrs after addition of glyoxal (100 fLg/ml) (e·----e OD260 ;
e-e counts per min)
in a 5-25% sucrose-gradient (e---·e OD260 ; e-e counts per min).
08
06
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gJ 04
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S28
•
''.
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e.,
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...
02.\
S4-6
T II
,I
,I
,I
,I
" ,~
II
'I
, I
, ,
, ,
1*\ I.: , I
! ,
,. , ,
I
•
O,--------~----~L-----~------~~
o
20
40
Fraction
60
80
1000
c::
E
a;
Q.
V)
C
:::J
0
U
500
b) Sedimentation profile of uri dine-labelled RNA (30 min pulse) in a 5-25 %
sucrose gradient 5 hrs after addition of glyoxal (100 fLg/ml) (e·----e OD260 ;
e-e counts per min)
