lnhibiton' Action of Radiolytic Compounds on Cell Function
181
o
en
GOO
40C
rS' Joe
n
200
20
30
Fraction
c) Comparison of the specific activity (counts/OD) of puiselabelled RNA after
1 hr treatment with 100 fLg/ml glyoxal ( 0 - 0 control; 6 - 6 glyoxal)
ished susceptibility to DNAse, strongly suggesting the inducement of
cross-links b\ irreversible binding of the dialdehyde to DNA through
non-electrostatic bonds. Analogous observations have been made with
DNA and ox\,dized polvamines [1].
These observations suggested an experiment to determine the temperature-absorption pattern of ON A from cells in culture after treatment with
glyoxal and malondialdeh\de and its susceptibility to DNAse. The DNA
isolated did, in fact, show an abnormal melting profile in comparison with
that of the untreated control cells and was only partially degraded by DNAse.
Since the treatment with the bifunctional aldehydes was too short (1 to
2 hrs) to interfere to any great extent with the synthesis of DNA, our results
would seem to substantiate the hypothesis of a primarily direct interaction.
References
1. BACHRACH, D., and G. EiLON: Biochem. Biophys. Acta 145, 418 (1967).
2. BERRY, R. ]., P. R. HILLS and W. TRILL WOOD : Int. J. Rad. BioI. 9, 559 (1965).
3. BROOKS, B. R., and O. L. KLA\!ERTH: in press.
4. CRAWFORD, D., R. 0, SINNlIlIBER, F. M. STOUT, J. E. OLDFIELD and J.
KAUH!ES: Toxicologv and Appl. Pharmacol. 7, 826 (1965).
5. CRAWFORD, D. L., T. c:. Yl' and O. R. SINNHUBER: J. of Food Science 32,
332 (1967).
6. DOERR, \'0'.: Verh. Deutsch. Gcs. Path. 32, 278 (1948).
7. GROS, F., \V. GILBERT, H, H. HIATT, G. ATTARD!, P. F. SPAHR and J. D.
WATSON: Cold Spring Harbour Symp. Quant. BioI. 26, 111 (1961).
8. HELGE, G.: Verh. Deutsch. Ges. Path. 42, 158 (1958).
8a. KLAMERTH, O. L.: Biochim. Biophys. Acta 155,271 (1968).
9. LEVINTHAJ., C, A. KEYNAN U. A. HIGA: Proc. Natl. Acad. Sci. US 48,1631,
(1962).
10. SAcHsp.n!AIER, \X., and D. IVES: Bioch. Z. 343, 399 (1965).
11. SCHAACK, M.: Z. gcs. exptl. NIcd. 135, 223 (1961).
181
o
en
GOO
40C
rS' Joe
n
200
20
30
Fraction
c) Comparison of the specific activity (counts/OD) of puiselabelled RNA after
1 hr treatment with 100 fLg/ml glyoxal ( 0 - 0 control; 6 - 6 glyoxal)
ished susceptibility to DNAse, strongly suggesting the inducement of
cross-links b\ irreversible binding of the dialdehyde to DNA through
non-electrostatic bonds. Analogous observations have been made with
DNA and ox\,dized polvamines [1].
These observations suggested an experiment to determine the temperature-absorption pattern of ON A from cells in culture after treatment with
glyoxal and malondialdeh\de and its susceptibility to DNAse. The DNA
isolated did, in fact, show an abnormal melting profile in comparison with
that of the untreated control cells and was only partially degraded by DNAse.
Since the treatment with the bifunctional aldehydes was too short (1 to
2 hrs) to interfere to any great extent with the synthesis of DNA, our results
would seem to substantiate the hypothesis of a primarily direct interaction.
References
1. BACHRACH, D., and G. EiLON: Biochem. Biophys. Acta 145, 418 (1967).
2. BERRY, R. ]., P. R. HILLS and W. TRILL WOOD : Int. J. Rad. BioI. 9, 559 (1965).
3. BROOKS, B. R., and O. L. KLA\!ERTH: in press.
4. CRAWFORD, D., R. 0, SINNlIlIBER, F. M. STOUT, J. E. OLDFIELD and J.
KAUH!ES: Toxicologv and Appl. Pharmacol. 7, 826 (1965).
5. CRAWFORD, D. L., T. c:. Yl' and O. R. SINNHUBER: J. of Food Science 32,
332 (1967).
6. DOERR, \'0'.: Verh. Deutsch. Gcs. Path. 32, 278 (1948).
7. GROS, F., \V. GILBERT, H, H. HIATT, G. ATTARD!, P. F. SPAHR and J. D.
WATSON: Cold Spring Harbour Symp. Quant. BioI. 26, 111 (1961).
8. HELGE, G.: Verh. Deutsch. Ges. Path. 42, 158 (1958).
8a. KLAMERTH, O. L.: Biochim. Biophys. Acta 155,271 (1968).
9. LEVINTHAJ., C, A. KEYNAN U. A. HIGA: Proc. Natl. Acad. Sci. US 48,1631,
(1962).
10. SAcHsp.n!AIER, \X., and D. IVES: Bioch. Z. 343, 399 (1965).
11. SCHAACK, M.: Z. gcs. exptl. NIcd. 135, 223 (1961).