- nested 220
- pair 209
prometaphase 11
promoter 17, 220, 321
- alternative 22
- metallothionein 322
- transthyretin 322
- unducible 322
pronucleus 323
- microinjection into 327
proof-reading 40
propidium iodide 170
protein
- biosynthesis 22
- purification 257
protein A-sepharose 373, 376
proteinase K 55, 56, 156
protein-protein interaction 284
pseudopregnant foster mothers
325
pSP6/T7 230
pUC 229, 291
- plamids 190
pulsed field gel electrophoresis
(see PFGE) 74
purification 50
R
RACE 219
random primers 280
- labeling 118
rare cutter 27
reading frame 295
rearrangements 219
recognition sequence 100
recombination 358
- fraction 359, 360
region of interest 207, 209
regulatory elements, cis-acting 321
Ren-2 gene 331
renin 322
repeat PCR, inter-alu 218
replication 16
- error rate 15
- fork 15
- origin of 228
reporter genes 322
retriction enzyrne,
recognition sites 217
SUBJECT INDEX
385
retroviral vectors 339
reverse transcriptase 44, 55, 219,
277,279
RFLPs 356
rhodamine 170
ribonuclease 60
riboprobes 121
ribosomal RNA (see rRNA) 77
ribosomes 17, 22
R-loop 17
RNA (ribonucleotide acid)
- in vitro transcription 121
- isolation of 60
- migration rate 77
- polymerase 17, 121
- rapid preparation 62, 64
- secondary structure of 8
- storage 70
- template 219
- total cellular 276
RNA blot hybridization 139
RNA dot blots 139
RNAse 78, 51
- block li 122
RNAse A 34
RNAse H 287
RüFE (rotating field inversion gel
electrophoresis) 103
rRNA (ribosomal RNA) 67, 77, 276
s
samples, paraffin embedded 218
satellite probe 169
second strand
- synthesis 261
- - cDNA 287
secondary structure 72, 204
sense strand 17
sequenase 42, 194
sequence
- difficult to clone 264
- information 209
- similarity 219, 354
- - cloning 257
- tagged sites (STSs) 219
sequencing
- automated fluoroscent 193
- chain termination 186, 192
- gel 200