384
SUBJECT INDEX
oligonucleotide 209, 257
- synthetiser 217
oocytes
- collection of 326
- Xenopus laevis (see also there)
312
orientation 295
oviduct transfer 327
p
Pl clones 99, 262
PACE (programmable autonomously-controlled electrodes)
97,99
pachytene 354, 355
packaging 279
- cell
- - infection of 350
- - line 341
- - transfection of 346
- extract 233
PAGE (polyacrylamide gel
electrophoresis) 73
partial digestion 100, 102
pBR322 229
PCR (polymerase chain reaction)
207pp.
- arbitrary primed 219
- competitive 222
- components of 207
- cycle 209
- in situ 219
- inverse 218
- jumping 218
- modifications 218
- multiplex 221
- one-sided 220
- protocol 211
- quantitative 222
- radioactive 221
- repeat 218
single cells 219
- specificity 214
- transposon-related 218
- troubleshooting 212
- yield 214
pepsin digestion 153
PFGE (pulsed field gel electrophoresis) 74, 96 pp., 103 pp.
- application of 99
- mapping 100
- pulse times 97
- resolution 97
- switch interval 97
A.phage 230
- A.gt10 230, 291
- A.gtll 230, 291
phage library 291
phagemid 297
phenol 50, 52
- salt-saturated 52
phenol/chloroform 50
- extraction 51
phosphodiester bond 6, 15, 209
physical mapping 103, 260
plaque density 296
plasmid 227
- library 290
- vectors 227
point mutations 221
poly{A) tail 17, 276
poly(AtRNA, preparation 67
polyacrylamide 73
- gel electrophoresis (PAGE) 73
polyacrylamide gels
- isolation of DNA 77
- nondenaturating 82
poly-L-lysine 157
polymerase 15
- activity 215
- chain reaction (see PCR) 207 pp.
- fidelity 215
- processivity 215
- thermostable 222
polymorphic DNA markers 356,
357
polymorphism 82
positional cloning 260, 354, 355
prehybridization 301
preparative gel 75
primer
- biotinylated 221
- concentration 217
- degenerate 217', 219, 220
- dimers 213
- extension 209
- fluorescence-labeled 221
- mismatch 217