- method 360
linkage 356, 358
- analysis 260, 354, 356
- - strategy of mapping
a disease gene 357
- significant 360
linker 279
- primers 220
- adapters 280
LOD score 358, 360
- curve 359
LODVIEW 362
lysis 230
- buffer 63
lytic cycle 230
M
M13 188,232
M13- derived cloning vectors 232
magnesium 215
magnetic beads,
streptavidin-coated 277
mammalian cells 57
manganese 222
marker locus 358
MCR (multiple cloning region)
190, 228
MDE 85
meiosis 13, 354
melting
- point 86
- temperature 216
membrane stripping 147
ß-mercaptoethanol 64
messenger RNA (see mRNA) 15
metaphase chromosome 5, 11
methyl mercuric hydroxide 78
methylase 280
methylation patterns 100
5-methyl-dCTP 280
microdissection 219
microsatellite marker 356,357,362
mineral oil 212
misincooperation 213
mismatch 127, 217
mispriming 217
mitochondrial genome 220
mitosis 13
mobility 71
SUBJECT INDEX
383
- zone of limiting 102
modification, posttranslational 17
molecular weight 71
- markers 75
Moloney Murine
- Leukemia Virus 340
- Sarcoma Virus 340
MoMulV 340
MOPS 79
3-morpholinopropanesulfonic
acid 79
mRNA (messanger RNA) 15, 276
- copies 281
multicolor fluorescence in situ
hybridization 182
multiple cloning region (MCR)
190, 228
mutations 260
- disease-causing 82
MVR-PCR 218
N
neomycin phospho-transferase
339
nick translation 118, 170, 171
nitrogenous bases 4
Northern
- blot 77, 140 pp., 281
- hybridization 67
nuclease Sl 33
- assays 67
nucleic acids 3, 4, 50
- extraction 51
nucleosides 4
nucleosome 11
nucleotide 4
- analogs 191
- diTP 191
- 7-deaza-dGTP 191
0
OD 54
OFAGE (orthogonal field agarose
gel electrophoresis) 98, 103
Okazaki fragments 15
OLA (oligomer ligation assay) 221
oligo(dT)
- cellulose 67, 69
- column 69