386
SUBJECT INDEX
- multiplexing 193
shearing 98
shuffle
- clones 214
- vectors 297
signal peptide 17
silencers 19
single
- cell suspensions from solid
tumor, preparation of 158
- strand
- - binding protein 204
- - conformation polymorphism
(see SSCP) 82
site-directed mutagenesis 220
size fractionation 271
sodium
- acetate 55
- lauryl sarkosinate 66
solenoid 11
somatic cell hybrids 262
Southern blot 131 pp.
spermine 55
S-phase 13
spin columns 76
splicing, alternative 17, 22
splicosomes 17
SSCP (single strand conformation
polymorphism) 82, 83, 221
- analysis 83
start codon 22
stem cells, embryonie 323
Stoffelfragment 222
stop codon 22
stringency 128
STSs (sequence tagged sites) 219
sucrose density gradient 271, 271
supercoiling 8
superhelix 8
superovulation 324
T
T4
- ligase 34
- polymerase 42
- polynucleotide kinase 114, 115
T7
- DNA polymerase 42, 194
- SP6 RNA polymerase 47
TAE 74
Taq DNA polymerase 43, 197, 209,
215
TBE 74
TE-buffer 57, 65
- RNAse-free 62
temperature
- annealing 216
- gradient gel electrophoresis
(TGGE) 221
template 207
terminal transferase 56, 114, 117
texas red 170
TGGE (temperature gradient gel
electrophoresis) 221
thimerosal 304
tissue samples 59
total RNA 67
tracking dye 74
transactivation 284
transcription 15, 17- 18
- factors 17, 18
- regulation of 20
transfection 230
transfer RNA (see tRNA) 8
transformation
- efficiencies 264, 283
- electroporation 238
- recombinant bacteriophage
DNA 241pp.
transgenic
- animals 321
- - hypertension research 330
- mice 324, 326
- rats 324, 325
- research, species in 329
translabel 3 71
translation 22
- mechanisms of 21
transport assay 315
transposon 218
- Tn5 339
tRNA 8, 23, 55, 67, 276
- structure of 10
- synthetase 23
trypsin-EDTA solution 154
two-hybrid 297
- system 258
type II restriction enzyme 26