380
SUBJECT INDEX
- division 13
- - cycle genes 15
- line
- - transfected 259
- - permanent 323
central dogma 17
CG
- buffer 80
- clamps 220
CHCL3
- remove 307
- tests 303
CHEF (contour clamped homogenous electric fields) 99, 103
chiasmata (see also crossing over)
354-356
chimera/chimerism 226, 273, 323
chimeric clones 279
chloroform 51
chloroform:isoamyl alcohol 50
chorionic villus biopsy 218
chromatids 354
chromatin 11
- structure of 12
chromosomal in situ suppression
( CISS) hybridization 179
chromosome 3, 11, 13
- bivalent 13
- homologaus 354
- metaphase 5, 11
- walking 262, 267, 269
CIP ( calf intestine alkaline
phosphatase) 36
cloning 226
- strategies 257 pp.
- vectors 226 pp.
codon 259
- usage 259
cohesive ends 279
colcemid solution 151
co-linearity 17, 19
co-migration 100
complexity 127
composite probe 169
confocallaser scanning
microscopy 183
contamination 213
cos sites 233, 279
cosmid 233, 261, 262
cRNA synthesis, in-vitro 316
crossing over (see also chiasmata)
354, 358
cyclin 15
cytogenetic abnormalities 260, 354
D
DAPI 170
ddNTPs 187
7-deaza-dGTP:dGTP 213
degeneracy 259
denaturating 72
- gradient gel electrophoresis
(DGGE) 221
denaturation 209, 212
deoxynucleotide triphosphates
(dNTPs) 209
DEPC (diethyl pyrocarbonate) 61
dephosphorylation 36
DGGE (denaturating gradient gel
electrophoresis) 221
- parallel 87
- perpendicular 87
diagnostic techniques 14
diethyl pyrocarbonate (DEPC) 61
differential
- cloning 260
- display 260, 295
digestion, partial 261, 271
digoxigenin 294
diploid genome 11
disease gene 354, 356
- genetic 257
DMSO 215
- shock 346
DNA (deoxynucleotide acid)
- biotinylation of 172
- circular 72
- complementary 261
- conformation 72
- degraded 218
- genomic 54, 259
- high-molecular-weight 50, 98,
103
- - genomic 54
- markers, known size 98
- microinjection of 326
- - foreign 323
- migration 71