- primary structure of 6, 7
- purification 50
- recovery of 75
- repair 15
- secondary structure of 6, 9
- superhelical 72
- tertiary structure of 10
DNA polymerase 14, 56, 190
- avian myeloblastosis virus
reverse transcriptase 191
- heat stable 209
- Klenow fragment 190
- T7 DNA polymerase 191
- Taq polymerase 191
DNAse I 33
dNTPS (deoxynucleotide
triphosphates) 209, 211, 215
- removal of 56
dot blot 129
- assay 174
- hybridization 221
- reverse 221
double
- digestion 32
- helix 6
dyad symmetry 8
E
E. coli HB101 234
EDTA 56
electrophoresis 71
- buffer 74
electroporation 238
EMBL 3 232
EMBL 4 232
end-labeling 38, 114
enhancers 19
epifluoroscence microscopy 170
ethanol 55
ethidium bromide 75
eukaryotic cells 50
- expression in 339
evolution 220
exchange kinase reaction 115, 116
exclusion mapping 361
exon-intron boundaries 17
exons 17
exonuclease activity 222
expression
- cloning 259, 312
- - examples of 318
- library 284, 291
- vectors 295
extension 212
- time 216
extraction buffer 58, 63
F
FIGE (field inversion gel
electrophoresis) 98, 103
fllling of 3' ends 40
SUBJECT INDEX
381
first strand cDNA, synthesis 286
FISH (fluoroescence in situ
hybridization) 148, 166
FITC 170
5' -flanking region 19
fluorochromes 170
footprinting 219
formaldehyde 78
formamide loading buffer 84
formamide-formaldehyde buffer
78
freeze-squeeze 76
fulllength cDNA 219
fusion proteins 295
G
GAL4 284
ßgalactosidase (lac-Z) gene 232,
322
gametogenesis 13
GC
- clamp 87
- content 216
gene 13
- expression 17
- - tissue-specific 19
- families 219
- knock-outs 262
- locus 358
- mapping 99
- product 17, 257
- transfer 339 pp.
genetic
- code 17, 22
- - degeneracy of 22, 257
- distance 358
information 15