Subject Index
A
acid citrate dextrose (ACD) 58
affinity chromatography,
oligo (dT) 274
agarose 73
- low melting point 76, 120
agarose/formaldehyde gel 78
allele-specific oligonucleotides
(ASOs) 221
alphoid probes 169
AMCA 170
amino acid sequence 257, 259
ammonium
- acetate 55
- ions 56
angiotensinogen 322
annealing 209, 212
anode 71
antibody
- choice 296
dilutions 296
- preadsorbtion 296
- secondary 296
antisense strand 17
ASOs (allele-specific
oligonucleotides) 221
autofluorescence 376
B
BACs (bacterial artificial
chromosomes) 99
BAP (bacterial alkaHne
phosphatase) 36
Barth's solution, modified 313
bases, complementary 207
ß-galactosidase gene 190
biohazard issues 351
biopanning 297
biotinylation of DNA 172
bivalents 354
blastocysts 323
blood
- samples 59
- smears 155
BLOTTO 377, 378
blue plaque 292
BNN97 302
bone marrow smears 156
break point 219
bromphenol blue 74
BTA282 302
c
CA repeat 356
ca++
- precipitation method 234
- transformation 234
canine microsomal membranes
372
5'-cap 17
carcinogen 61
CAT (chloramphenicol-acetyltransferase) gene 322
CCD (charge-coupled device)
camera 170
cdc2 kirrase 15
cDNA 275pp.
- expression library 315
- insert 279
- library 257, 275
- - vector selection 283
- single stranded 277
cell
- cycle 13, 15