366
L. Pellerito . R. Barbieri· R. Di Stefano . M. Scopelliti . C. Pellerito . T. Fiore . F. Triolo
a
b
c
Fig. 15.23. Phagocytizing haemocytes, showing; a positivity for Ca2+ -ATPase (arrowhead); b localized
internal calcium rise in normal conditions (arrowhead); c diffuse internal calcium rise in the presence
of TBT. Bars: 10 f1Il1, adapted from Cima et al. (1995)
Fig. 15.24. Phagocytosis of
Ciona intestinalis haemocytes
after incubation (90 min) with
different concentrations of
organotin compounds;
(a) 1.51LM, (b) 0.15 fLM,
(c) 0.015 ILM, (d) 0.0015 fLM
and (e) MS = marine solution,
adapted from Cooper et al. (1995)
so
l
'"
.~ 40
>. u o
C\
'"
..c
IJ. 30
'0
C\
!9 I:
~ 20
~
10
o
TBT
DBT
TPT
DPT
MS
An ultrastructural study carried out by Gianguzza et al. (1996), by exposing embryos of the asci dian Ciona intestinalis to tributyltin(IV)chloride solutions, at two
different stages of development (1) before hatching (coiled larval stage) and (2) 2 h
after hatching (swimming larval stage) led to the following conclusions: