Table 2 Diversity indices (PAST software)
based on DGGE profile of bacterial population present in sediment and water samples
in Sidi Fredj Port
Sediment
Seawater
Sed1
Sed2
Sed3
Sed4
Sw1
Sw2
Sw3
Sw4
Taxa_S
20
11
12
7
27
30
31
19
Shannon
2Á996
2Á398
2Á485
1Á946
3Á296
3Á401
3Á434
2Á944
Chao-1
210
66
78
28
378
465
496
190
Table 3 Identification of bacterial strains isolated from Sidi Fredj Port sediment and seawater samples (Sw: Seawater samples, Sed: sediment
samples)
Isolate
code
Station
Phylogeny
Blast 16S
Phylum
Class
Family
Closest relative
Id.
(%)
Accession
number
PSF-5Sw
Sw3
Actinobacteria Actinobacteria
Brevibacteriaceae
Brevibacterium celere strain KMM
3637
99 NR_025727.1
PSF-6S
Sed1
Gordoniaceae
Gordonia hongkongensis strain
HKU50
99 NR_152023.1
PSF-23S
Sed3
Micrococcaceae
Micrococcus yunnanensis strain YIM
65004
99 NR_116578.1
PSF-8Sw
Sw5
Bacteroidetes
Flavobacteriia
Flavobacteriaceae
Xanthomarina gelatinilytica strain
AK20
100 NR_145579.1
PSF-14Sw Sw2
Proteobacteria Alphaproteobacteria
Erythrobacteraceae Erythrobacter citreus strain RE35F/1
99 NR_028741.1
PSF-16Sw Sw5
Rhodobacteraceae
Labrenzia aggregata strain NBRC
16684
99 NR_113861.1
PSF-12Sw Sw3
Gammaproteobacteria Alcanivoracaceae
Alcanivorax borkumensis strain SK2
99 NR_074890.1
PSF-90Sw Sw1
Alcanivorax borkumensis strain SK2
99 NR_074890.1
PSF-88Sw Sw4
Alcanivorax borkumensis strain SK2
99 NR_074890.1
PSF-3Sw
Sw5
Alcanivorax borkumensis strain SK2
100 NR_074890.1
PSF-84S
Sed2
Alcanivorax borkumensis strain SK2
99 NR_074890.1
PSF-30Sw Sw1
Alcanivorax xenomutans strain
JC109
99 NR_133958.1
PSF-34Sw Sw3
Alcanivorax xenomutans strain
JC109
99 NR_133958.1
PSF-54S
Sed3
Alcanivorax xenomutans strain
JC109
100 NR_133958.1
PSF-18S
Sed4
Alcanivorax dieselolei strain B5
99 NR_074734.1
PSF-13Sw Sw3
Halomonadaceae
Halomonas venusta strain NBRC
102221
99 NR_114048.1
PSF-31S
Sed3
Alteromonadaceae
Marinobacter hydrocarbonoclasticus
strain
ATCC 49840
99 NR_074619.1
PSF-26Sw Sw1
Marinobacter hydrocarbonoclasticus
strain
ATCC 49840
99 NR_074619.1
PSF-42S
Sed1
Marinobacter nitratireducens strain
AK21
99 NR_136469.1
PSF-43Sw Sw3
Marinobacter nitratireducens strain
AK21
99 NR_136469.1
PSF-15Sw Sw2
Marinobacter zhanjiangensis strain
JSM 078120
99 NR_116657.1
PSF-21Sw Sw3
Pseudomonadaceae Pseudomonas oceani strain DSM
100277
99 NR_152090.1
PSF-35Sw Sw1
Pseudomonas taiwanensis strain
BCRC 17751
99 NR_116172.1
Journal of Applied Microbiology 126, 780--795 © 2018 The Society for Applied Microbiology
787
N. Djahnit et al.
The use of oil-degrading bacteria as an alternative for environmental remediation (bioremediation)