on crude oil and n-hexadecane as carbon source with
diverse yields, except to strain PSF-16Sw that was able to
grow only on crude oil and benzene. Ten isolates were
able to grow on all aliphatic hydrocarbons tested (nhexadecane, n-hexacosane and n-octacosane); however,
none of them grew on medium amended with aromatic
hydrocarbons. These isolates are affiliated to Alcanivorax
(PSF-3Sw, PSF-12Sw, PSF-30Sw, PSF-18S, PSF-54S, and
1·00E+00
1·00E+01
1·00E+02
1·00E+03
1·00E+04
1·00E+05
1·00E+06
Sw1
Sw2
Sw3
Sw4
Sw5
DAPI Count (Log Cell ml –1
)
Figure 2 Microbial abundance measured as DAPI total counts (cell ml
À1
) verified in seawater samples of Sidi Fredj port.
Similarity
(a)
(b)
1·0
100
79
SW1
SW2
SW3
SW4
Sed2
Sed3
Sed1
Sed4
77
60
35
39
100
0·9
0·8
0·7
0·6
0·5
0·3
0·4
0·2
Figure 3 Bacterial diversity of seawater and sediment of Sidi Fredj Port. (a) Cluster analysis dendrogram displaying the bacterial community of seawater and sediment samples. Jaccard’s coefficient and 100 bootstrap analysis were used to measure similarity among the eight DGGE banding patterns. (b) DGGE profiles of PCR amplified 16S rRNA V3 region from seawater and surface sediment samples. Sw5 and sed5 samples are missing.
[Colour figure can be viewed at wileyonlinelibrary.com]
Journal of Applied Microbiology 126, 780--795 © 2018 The Society for Applied Microbiology
786
The use of oil-degrading bacteria as an alternative for environmental remediation (bioremediation)
N. Djahnit et al.