about of acetic acid or sodium acetate is added at the beginning of the culture
process, and small additional amounts are supplied using the medium’s rising pH as
an indicator. Carbon is a major component accounting for 51.4–72.6% of the leaf
Table 7.4 Medium used in mass culturing
Medium Ingredients (g/L)
A
B
C
D
E
Potassium nitrate (KNO 3 )
5.0
1.25
Urea
3.0
6.0
1.5
Monopotassium phosphate (KH 2 PO 4)
1.25
1.25
1.25
1.0
1.2
Magnesium sulfate (MgSO 4 Á7H 2 O)
2.5
2.5
1.25
1.0
0.6
Calcium chloride (CaCl 2 )
0.03
Ferrous sulfate (FeSO 4 Á7H 2 O)
0.003
0.003
0.02
0.015
EDTA-Na–Fe
0.015
EDTA-Na 2
0.037
A 5 solution (mL)
a
1.0
3.0
2.0
Trace elements
A 4 ÁB 7
b
Citric acid
0.25
Glucose
20
Acetic acid (mL)
c
100
a Dissolve 2.86 g H 3 BO 3 , 1.81 g MgCl 2 Á4H 2 O, 0.22 g ZnSO 4 Á7H 2 O, 0.08 g CuSO 4 Á5H 2 O, and
0.021 Na 2 MoO 4 in 1L of pure water and add one drop of H 2 SO 4
b
Add 1.0 mL solution containing 0.6 ppm Fe, 0.5 ppm B and Mn, 0.05 ppm Zn, 0.02 ppm Cu,
and 0.1 ppm Mo, Co, Ni, Cr, V, W, and Ti
c Add small quantities according to amount consumed
Fig. 7.4 Relationship between culturing rate and specific growth rate for multiple strains
7.3 Mass-Production of Microalgae
209