Also drawing attention is the use of auxins and cytokines that mediate plant
growth in the culturing of seaweed tissue. Indole acetic acid and kinetine have
proven effective when mixed in sea lettuces (a form of brown algae), as have phenyl
acetic acid, zeatin, 2,4,5-trichlorophenoxy acetic acid, 6-benzylaminopurine, indole
acetic acid, and kinetine when mixed with the red alga Agardhiella. Research on the
Table 6.5 Suitable temperatures and light intensities for callus proliferation and sporophyte
differentiation in gonidium tissue from seaweeds in kelp order. (1) Cut tissue fragment. (2) Wipe
with paper towel and remove surface portion. (3) Dip in 100% ethanol for 1 to 2 s. (4) Sear surface
with burner. (5) Cut to 3–4 mm. (6) Place on agar medium (Notoya 2000)
Species
Callus cell multiplication
Leaf body cell differentiation
Agarum cribrosum
10 °C, 10–80 µ mol m
−2
s
−1
–
Costaria costata
15 °C, 10–40 µ mol m
−2
s
−1
15 °C, 80 µ mol m
−2
s
−1
20 °C, 40–80 µ mol m
−2
s
−1
15 °C, 10–20 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
15 °C, 40–80 µ mol m
−2
s
−1
20 °C, 10–80 µ mol m
−2
s
−1
Ecklonia cava
15 °C, 30–32 µ mol m
−2
s
−1
15 °C, 30–32 µ mol m
−2
s
−1
20 °C, 10–20 µ mol m
−2
s
−1
15 °C, 40–80 µ mol m
−2
s
−1
20 °C, 40–80 µ mol m
−2
s
−1
25 °C, 10–80 µ mol m
−2
s
−1
15 °C, 10–20 µ mol m
−2
s
−1
20 °C, 80 µ mol m
−2
s
−1
20 °C, 10–80 µ mol m
−2
s
−1
Ecklonia stolonifera
10 °C, 10–40 µ mol m
−2
s
−1
15 °C, 80 µ mol m
−2
s
−1
15 °C, 10–40 µ mol m
−2
s
−1
20 °C, 20–80 µ mol m
−2
s
−1
25 °C, 10–40 µ mol m
−2
s
−1
Eisenia bicyclis
20 °C, 10 µ mol m
−2
s
−1
15 °C, 20–80 µ mol m
−2
s
−1
20 °C, 20–80 µ mol m
−2
s
−1
25 °C, 10–80 µ mol m
−2
s
−1
20 °C, 10 µ mol m
−2
s
−1
10 °C, 40–80 µ mol m
−2
s
−1
15 °C, 40–80 µ mol m
−2
s
−1
20 °C, 20–80 µ mol m
−2
s
−1
25 °C, 20–80 µ mol m
−2
s
−1
Kjellmaniella crassifolia
10 °C, 10 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
Undaria pinnatifida
15 °C, 10 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
15 °C, 20–80 µ mol m
−2
s
−1
20 °C, 20–40 µ mol m
−2
s
−1
15 °C, 10 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
15 °C, 80 µ mol m
−2
s
−1
20 °C, 40 µ mol m
−2
s
−1
6.3 Tissue Culture
155
growth in the culturing of seaweed tissue. Indole acetic acid and kinetine have
proven effective when mixed in sea lettuces (a form of brown algae), as have phenyl
acetic acid, zeatin, 2,4,5-trichlorophenoxy acetic acid, 6-benzylaminopurine, indole
acetic acid, and kinetine when mixed with the red alga Agardhiella. Research on the
Table 6.5 Suitable temperatures and light intensities for callus proliferation and sporophyte
differentiation in gonidium tissue from seaweeds in kelp order. (1) Cut tissue fragment. (2) Wipe
with paper towel and remove surface portion. (3) Dip in 100% ethanol for 1 to 2 s. (4) Sear surface
with burner. (5) Cut to 3–4 mm. (6) Place on agar medium (Notoya 2000)
Species
Callus cell multiplication
Leaf body cell differentiation
Agarum cribrosum
10 °C, 10–80 µ mol m
−2
s
−1
–
Costaria costata
15 °C, 10–40 µ mol m
−2
s
−1
15 °C, 80 µ mol m
−2
s
−1
20 °C, 40–80 µ mol m
−2
s
−1
15 °C, 10–20 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
15 °C, 40–80 µ mol m
−2
s
−1
20 °C, 10–80 µ mol m
−2
s
−1
Ecklonia cava
15 °C, 30–32 µ mol m
−2
s
−1
15 °C, 30–32 µ mol m
−2
s
−1
20 °C, 10–20 µ mol m
−2
s
−1
15 °C, 40–80 µ mol m
−2
s
−1
20 °C, 40–80 µ mol m
−2
s
−1
25 °C, 10–80 µ mol m
−2
s
−1
15 °C, 10–20 µ mol m
−2
s
−1
20 °C, 80 µ mol m
−2
s
−1
20 °C, 10–80 µ mol m
−2
s
−1
Ecklonia stolonifera
10 °C, 10–40 µ mol m
−2
s
−1
15 °C, 80 µ mol m
−2
s
−1
15 °C, 10–40 µ mol m
−2
s
−1
20 °C, 20–80 µ mol m
−2
s
−1
25 °C, 10–40 µ mol m
−2
s
−1
Eisenia bicyclis
20 °C, 10 µ mol m
−2
s
−1
15 °C, 20–80 µ mol m
−2
s
−1
20 °C, 20–80 µ mol m
−2
s
−1
25 °C, 10–80 µ mol m
−2
s
−1
20 °C, 10 µ mol m
−2
s
−1
10 °C, 40–80 µ mol m
−2
s
−1
15 °C, 40–80 µ mol m
−2
s
−1
20 °C, 20–80 µ mol m
−2
s
−1
25 °C, 20–80 µ mol m
−2
s
−1
Kjellmaniella crassifolia
10 °C, 10 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
Undaria pinnatifida
15 °C, 10 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
15 °C, 20–80 µ mol m
−2
s
−1
20 °C, 20–40 µ mol m
−2
s
−1
15 °C, 10 µ mol m
−2
s
−1
10 °C, 80 µ mol m
−2
s
−1
15 °C, 80 µ mol m
−2
s
−1
20 °C, 40 µ mol m
−2
s
−1
6.3 Tissue Culture
155