(1) Cut tissue fragment. (2) Wipe with paper towel and remove surface portion.
(3) Dip in 100% ethanol for 1 to 2 seconds. (4) Sear surface with burner.
(5) Cut to 3–4mm. (6) Place on agar medium.
Fig. 6.2 Tissue culturing sequence for Ecklonia stolonifera (Aruga 1990)
Table 6.4 Seaweeds currently used for tissue culturing (Polne-Fuller and Gibor 1987;
Polne-Fuller 1988; Amano 1990)
Green algae
Brown algae
Red algae
Bryopsis plumosa
Dictyosiphon foeniculaceus
Porphyra ochotensis
Ulva linza (Green laver)
Ecklonia stolonifera
Porphyra
yezoensis
(Nori)
Ulva intestinalis
(Green laver)
Laminariaceae (Kelp)
Agardhiella subulata
Kornmannia leptoderma
(Green laver)
Laminaria japonica (Japanese seatangle)
Chondrus crispus
Monostroma angicava
(Green laver)
Laminaria angustata
Gelidiaceae
Ulva taniata (Green laver)
Laminaria saccharina
Gelidium amansii
(Long limu)
s
i
t
s
y
c
o
r
c
a
M
pyrifera (Giant kelp)
Pterocladiella capillacea
Undaria pinnatifida
Gelidium divaricatum
Sargassum heterophyllum
Gigartina exasperata
s
u
h
t
n
a
c
a
r
d
n
o
h
C
tenellus
Gigartinaceae
Gracilaria deblis
Gracilaria epihippisora
Gracilaria tikvahiae
154
6 Seaweed Biotechnology