233
8 Two-Dimensional Mid-Infrared Correlation Spectroscopy in Protein Research
been postulated that the presence of β-lg makes the urea dimers less stable and more
sensitive to changes in the urea concentration.
8.4 Developments in Generalized 2DCoS Useful
in Protein Research
8.4.1 Heterospectral Correlation
the summary plot in Fig. 8.7 shows the various optical spectroscopies that have
been combined with 2dCoS to provide complimentary information about protein
structure at different levels of organization and interaction with environment. Such
a large range of methods involved in protein studies has opened a new possibility for 2dCoS called heterospectral correlation analysis. heterospectral correlation
analysis involves comparison of the spectral changes induced by identical perturbations and measured by closely related spectroscopic methods. this approach can
also be involved in combined analyses of the results obtained from completely dif1300
1400
1500
1600
1700
1300
1400
1500
1600
1700
ν
2 , wavenumber (cm -1
) - urea
ν 1 , wavenumber (cm -1 ) – urea/β-lg
0
1563
1465
Slice@1562 cm -1
Fig. 8.6 heterosample
asynchronous 2d correlation
spectrum for the two systems
and the slice spectrum
extracted at the position of
their maximal asynchronicity
shown above. the solid and
dashed lines represent positive and negative cross peaks,
respectively. (Reproduced
with permission from Ref.
No. 30. Copyright (2009)
American Chemical Society)
8 Two-Dimensional Mid-Infrared Correlation Spectroscopy in Protein Research
been postulated that the presence of β-lg makes the urea dimers less stable and more
sensitive to changes in the urea concentration.
8.4 Developments in Generalized 2DCoS Useful
in Protein Research
8.4.1 Heterospectral Correlation
the summary plot in Fig. 8.7 shows the various optical spectroscopies that have
been combined with 2dCoS to provide complimentary information about protein
structure at different levels of organization and interaction with environment. Such
a large range of methods involved in protein studies has opened a new possibility for 2dCoS called heterospectral correlation analysis. heterospectral correlation
analysis involves comparison of the spectral changes induced by identical perturbations and measured by closely related spectroscopic methods. this approach can
also be involved in combined analyses of the results obtained from completely dif1300
1400
1500
1600
1700
1300
1400
1500
1600
1700
ν
2 , wavenumber (cm -1
) - urea
ν 1 , wavenumber (cm -1 ) – urea/β-lg
0
1563
1465
Slice@1562 cm -1
Fig. 8.6 heterosample
asynchronous 2d correlation
spectrum for the two systems
and the slice spectrum
extracted at the position of
their maximal asynchronicity
shown above. the solid and
dashed lines represent positive and negative cross peaks,
respectively. (Reproduced
with permission from Ref.
No. 30. Copyright (2009)
American Chemical Society)