process that is the relevant transition in fireflies. Future work recording the light
emitted from electronically excited oxyluciferin anions isolated in vacuo would
therefore be highly illuminating. Also the quantum yield for light emission when
there is no protein environment would be relevant to measure, and whether one
single water molecule plays a biological role or not.
References
1. Lakowicz, J.R.: Principles of Fluorescence Spectroscopy, 2nd edn. Kluwer Academic/Plenum,
New York (1999)
2. Forbes, M.W., Jockusch, R.A.: Gas-phase fluorescence excitation and emission spectroscopy
of three xanthene dyes (Rhodamine 575, Rhodamine 590 and Rhodamine 6G) in a quadrupole
ion trap mass spectrometer. J. Am. Soc. Mass Spectrom. 22, 93–109 (2011)
3. Bian, Q., Forbes, M.W., Talbot, F.O., Jockusch, R.A.: Gas-phase fluorescence excitation and
emission spectroscopy of mass-selected trapped molecular ions. Phys. Chem. Chem. Phys. 12,
2590–2598 (2010)
4. Friedrich, J., Fu, J., Hendricksson, C.L., Marshall, A.G., Wang, Y.-S.: Time resolved laserinduced fluorescence of electrosprayed ions confined in a linear quadrupole trap. Rev. Sci.
Instrum. 75, 4511–4515 (2004)
5. Khoury, J.T., Rodriguez-Cruz, S.E., Parks, J.H.: Pulsed fluorescence measurements of trapped
molecular ions with zero background detection. J. Am. Soc. Mass Spectrom. 13, 696–708
(2002)
Fig. 6.9 Action spectra of
(a) bare meta-nitrophenolate
anion, (b) anion with one
water attached, (c) anion with
one methanol attached, and
(d) anion with one acetonitrile
attached. The red sticks are
CC2-calculated values. The
blue dashed curve in (d) is the
spectrum obtained in bulk
acetonitrile solution.
Distances [in A ˚ ] from
hydrogen to the phenolate
oxygen are given on the
structures to the right. Carbon
is grey, oxygen red, nitrogen
blue, and hydrogen white.
Reprinted with permission
from [29]. Copyright (2013)
American Chemical Society
114
S.B. Nielsen
emitted from electronically excited oxyluciferin anions isolated in vacuo would
therefore be highly illuminating. Also the quantum yield for light emission when
there is no protein environment would be relevant to measure, and whether one
single water molecule plays a biological role or not.
References
1. Lakowicz, J.R.: Principles of Fluorescence Spectroscopy, 2nd edn. Kluwer Academic/Plenum,
New York (1999)
2. Forbes, M.W., Jockusch, R.A.: Gas-phase fluorescence excitation and emission spectroscopy
of three xanthene dyes (Rhodamine 575, Rhodamine 590 and Rhodamine 6G) in a quadrupole
ion trap mass spectrometer. J. Am. Soc. Mass Spectrom. 22, 93–109 (2011)
3. Bian, Q., Forbes, M.W., Talbot, F.O., Jockusch, R.A.: Gas-phase fluorescence excitation and
emission spectroscopy of mass-selected trapped molecular ions. Phys. Chem. Chem. Phys. 12,
2590–2598 (2010)
4. Friedrich, J., Fu, J., Hendricksson, C.L., Marshall, A.G., Wang, Y.-S.: Time resolved laserinduced fluorescence of electrosprayed ions confined in a linear quadrupole trap. Rev. Sci.
Instrum. 75, 4511–4515 (2004)
5. Khoury, J.T., Rodriguez-Cruz, S.E., Parks, J.H.: Pulsed fluorescence measurements of trapped
molecular ions with zero background detection. J. Am. Soc. Mass Spectrom. 13, 696–708
(2002)
Fig. 6.9 Action spectra of
(a) bare meta-nitrophenolate
anion, (b) anion with one
water attached, (c) anion with
one methanol attached, and
(d) anion with one acetonitrile
attached. The red sticks are
CC2-calculated values. The
blue dashed curve in (d) is the
spectrum obtained in bulk
acetonitrile solution.
Distances [in A ˚ ] from
hydrogen to the phenolate
oxygen are given on the
structures to the right. Carbon
is grey, oxygen red, nitrogen
blue, and hydrogen white.
Reprinted with permission
from [29]. Copyright (2013)
American Chemical Society
114
S.B. Nielsen