5.14 Studies of Spectral Features of Radiation Scattered by Blood …
465
For conducting experiments, blood preparation of rabbits was used, which was
preliminary prepared by adding heparin to it for preventing coagulability and subsequent centrifugation with the aim of elimination of plasma, white blood cell, etc.
Hermetically closed cuvette with homogeneous aqueous solution of red blood cells
was placed into the compartment for the test of probes of spectrophotometer, and
cuvette with distilled water—on the place of comparison probe.
The spectra of blood absorption were registered, on which characteristic local
extremums on three wavelengths of λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577 nm
were observed, and their amplitudes depended on the degree of blood oxygenation.
Simultaneously with conducting of these experiments, the protein concentration
in probes under study was determined by biuret method.
The conducted experimental studies of absorption spectra of blood preparation
during changes of concentration of oxyhemoglobin allowed concluding that changes
of amplitudes of typical local extremums of spectral curve at wavelengths of 542, 560,
577 nm are connected with the level of blood oxygenation. Registration of spectral
curves of reflection coefficient at different degrees of artificially caused ischemia
of skin allowed stating the fact that the increase of the degree of ischemia leads to
gradual distinction of local extremums of spectral curve at wavelengths of 542 and
577 nm and change of a sign of curvature at a wavelength of 560 nm.
Correspondingly, there takes place an increase of suggested coefficients, which
are responsible for the differences between the amplitudes of local extremums at
wavelengths of 542 and 547 nm and the amplitude for a wavelength of 560 nm and
depending on the concentration of oxyhemoglobin in blood.
For the estimation of influence of ILBI on the change of the concentration of
oxyhemoglobin, laser radiation of blood via placing optical fiber into the auricle of
a rabbit was conducted. The exposure time during this process amounted to 10 min
at the radiation power at the exit of fiber that equals to 4 mW. He–Ne laser of LG-79
(λ = 632.8 nm) type was used for the radiation. The course of ILBI was conducted
for 10 days (10 procedures, 10 min each).
Spectral properties of radiation scattered by the preparations of blood of experimental animals were studied after 1 month after conducting the course of ILBI
therapy. Animals with damages of peripheral nerve served as control, and the course
of ILBI therapy was not conducted on them.
Based on the described technique, blood preparation after ILBI was prepared
and absorption spectrum with the help of spectrophotometer “Specord M 40” was
registered. At the spectral curves, there were fixed the changes of amplitudes of
characteristic local extremums on three wavelengths of 542, 560 and 577 nm, which
were obviously caused by the influence of laser radiation.
Spectral curves of absorption spectra of native preparations of blood of experimental animals before and after the course of ILBI with the help of He–Ne laser are
presented in Fig. 5.64.
For the quantitative estimation of concentration of oxyhemoglobin in blood preparation of experimental animals before and after conducting ILBI and for the normalization of the results of the measurements, the difference of the calculated ratios
of the amplitudes of characteristic local extremums, registered spectral curves of
465
For conducting experiments, blood preparation of rabbits was used, which was
preliminary prepared by adding heparin to it for preventing coagulability and subsequent centrifugation with the aim of elimination of plasma, white blood cell, etc.
Hermetically closed cuvette with homogeneous aqueous solution of red blood cells
was placed into the compartment for the test of probes of spectrophotometer, and
cuvette with distilled water—on the place of comparison probe.
The spectra of blood absorption were registered, on which characteristic local
extremums on three wavelengths of λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577 nm
were observed, and their amplitudes depended on the degree of blood oxygenation.
Simultaneously with conducting of these experiments, the protein concentration
in probes under study was determined by biuret method.
The conducted experimental studies of absorption spectra of blood preparation
during changes of concentration of oxyhemoglobin allowed concluding that changes
of amplitudes of typical local extremums of spectral curve at wavelengths of 542, 560,
577 nm are connected with the level of blood oxygenation. Registration of spectral
curves of reflection coefficient at different degrees of artificially caused ischemia
of skin allowed stating the fact that the increase of the degree of ischemia leads to
gradual distinction of local extremums of spectral curve at wavelengths of 542 and
577 nm and change of a sign of curvature at a wavelength of 560 nm.
Correspondingly, there takes place an increase of suggested coefficients, which
are responsible for the differences between the amplitudes of local extremums at
wavelengths of 542 and 547 nm and the amplitude for a wavelength of 560 nm and
depending on the concentration of oxyhemoglobin in blood.
For the estimation of influence of ILBI on the change of the concentration of
oxyhemoglobin, laser radiation of blood via placing optical fiber into the auricle of
a rabbit was conducted. The exposure time during this process amounted to 10 min
at the radiation power at the exit of fiber that equals to 4 mW. He–Ne laser of LG-79
(λ = 632.8 nm) type was used for the radiation. The course of ILBI was conducted
for 10 days (10 procedures, 10 min each).
Spectral properties of radiation scattered by the preparations of blood of experimental animals were studied after 1 month after conducting the course of ILBI
therapy. Animals with damages of peripheral nerve served as control, and the course
of ILBI therapy was not conducted on them.
Based on the described technique, blood preparation after ILBI was prepared
and absorption spectrum with the help of spectrophotometer “Specord M 40” was
registered. At the spectral curves, there were fixed the changes of amplitudes of
characteristic local extremums on three wavelengths of 542, 560 and 577 nm, which
were obviously caused by the influence of laser radiation.
Spectral curves of absorption spectra of native preparations of blood of experimental animals before and after the course of ILBI with the help of He–Ne laser are
presented in Fig. 5.64.
For the quantitative estimation of concentration of oxyhemoglobin in blood preparation of experimental animals before and after conducting ILBI and for the normalization of the results of the measurements, the difference of the calculated ratios
of the amplitudes of characteristic local extremums, registered spectral curves of