464
5 Laser Specklometer, Speckle-Optical Diagnostics and Laser …
5.14 Studies of Spectral Features of Radiation Scattered
by Blood Preparations and Skin of Human Being
and Animals at ILBI
In our studies [56–58, 265–269], it has been experimentally established that at traumatic and ischemic damages of peripheral nerves ILBI therapy causes normalization
of prooxidant reactions, enzymatic and hormonal disorders, increase of the level
of immune active proteins and the activity of antioxidant of system, improvement
of functional state of neuromotor apparatus and microhemocirculation that leads to
the enforcement of compensatory adaptive reactions of organism in the conditions
of simulating pathology [165, 166]. Consequently, ILBI influencing the circulating
blood has biostimulating effect, which normalizes the condition of the main functional systems and organs that allows applying this kind of non-medicament therapy
at different pathologies, including the diseases of nervous system: multiple sclerosis,
cerebrovascular pathology, disease of peripheral nervous system [169].
The information about the level of skin chromophores, hemoglobin and oxyhemoglobin in skin blood flow has importance for diagnostics of hypoxic changes in
tissues at damages of periphery nerves [83, 265, 266, 278, 282, 283, 284].
Spectral analysis is one of the effective methods of getting this information. The
studies of the absorption spectra of blood preparation of animals with the help of
three-wavelength spectrophotometric method at ILBI allow determining the influence of low-intensity laser irradiation (LILI) on such a significant index of oxygen
transfer–blood function, as a degree of oxygenation, and using this criterion as a
test for the objectification of the disorders of oxygen-transfer blood function, at
different variants of damages of peripheral nerves (traumatic damages of nerve trunks
and partial ischemia of back limb of animals with the development of ischemic
neuropathy of sciatic nerve), also they allow studying the possibility of correction of
this indicator via course of ILBI.
5.14.1 Study of Influence of ILBI on Spectral Properties
of Radiation Scattered by Blood Preparation
of Animals with the Help of Three-Wavelength
Spectrophotometric Method
The experiments based on the studies of the changes of spectral properties of radiation
scattered by blood preparation of rabbits in a range of 200–900 nm before and
after conducting the course ILBI were carried out on 37 rabbits of both sexes at
norm, at traumatic damage of peripheral nerves and partial ischemia of peripheral
nerve of animals. The studies were conducted with the help of special attachment
to the spectrophotometer “Specord M 40” for the measurement of transmission and
absorption.
5 Laser Specklometer, Speckle-Optical Diagnostics and Laser …
5.14 Studies of Spectral Features of Radiation Scattered
by Blood Preparations and Skin of Human Being
and Animals at ILBI
In our studies [56–58, 265–269], it has been experimentally established that at traumatic and ischemic damages of peripheral nerves ILBI therapy causes normalization
of prooxidant reactions, enzymatic and hormonal disorders, increase of the level
of immune active proteins and the activity of antioxidant of system, improvement
of functional state of neuromotor apparatus and microhemocirculation that leads to
the enforcement of compensatory adaptive reactions of organism in the conditions
of simulating pathology [165, 166]. Consequently, ILBI influencing the circulating
blood has biostimulating effect, which normalizes the condition of the main functional systems and organs that allows applying this kind of non-medicament therapy
at different pathologies, including the diseases of nervous system: multiple sclerosis,
cerebrovascular pathology, disease of peripheral nervous system [169].
The information about the level of skin chromophores, hemoglobin and oxyhemoglobin in skin blood flow has importance for diagnostics of hypoxic changes in
tissues at damages of periphery nerves [83, 265, 266, 278, 282, 283, 284].
Spectral analysis is one of the effective methods of getting this information. The
studies of the absorption spectra of blood preparation of animals with the help of
three-wavelength spectrophotometric method at ILBI allow determining the influence of low-intensity laser irradiation (LILI) on such a significant index of oxygen
transfer–blood function, as a degree of oxygenation, and using this criterion as a
test for the objectification of the disorders of oxygen-transfer blood function, at
different variants of damages of peripheral nerves (traumatic damages of nerve trunks
and partial ischemia of back limb of animals with the development of ischemic
neuropathy of sciatic nerve), also they allow studying the possibility of correction of
this indicator via course of ILBI.
5.14.1 Study of Influence of ILBI on Spectral Properties
of Radiation Scattered by Blood Preparation
of Animals with the Help of Three-Wavelength
Spectrophotometric Method
The experiments based on the studies of the changes of spectral properties of radiation
scattered by blood preparation of rabbits in a range of 200–900 nm before and
after conducting the course ILBI were carried out on 37 rabbits of both sexes at
norm, at traumatic damage of peripheral nerves and partial ischemia of peripheral
nerve of animals. The studies were conducted with the help of special attachment
to the spectrophotometer “Specord M 40” for the measurement of transmission and
absorption.