462
5 Laser Specklometer, Speckle-Optical Diagnostics and Laser …
determined by individual reflecting characteristics of skin of every single respondent.
Thus, according to our data, the method of the diagnostics of ischemia suggested by
the authors is not reliable due to a great influence on the results of measurements of
individual characteristics of reflecting features of human skin.
The study of spectral reflection coefficient of skin in vivo on the inner side of a
thumb was conducted at different functional states. The measurements of spectral
curves of reflection coefficient were conducted with the use of the attachment with
integrating sphere photometer at two people. People under test tightly applied to the
aperture for installation of a sample of integrating sphere photometer the area under
study of skin at the inner side of a thumb. Spectral changes of reflection coefficient of
skin at the same conditions (Fig. 5.62, lines 1 and 2 for both testees) were registered.
Artificial ischemia of the skin area was caused by air inflating of air-inflated collar,
which had been put on forearm beforehand, till complete blood flow stops of a limb.
Later, the changes of spectral reflection coefficients of skin were registered, which
are presented in Fig. 5.62 (the line 3 for the first testee and the line 4 for the second
one). Then, a special cuvette with blood preparation was installed into the aperture
for the installation of a sample of integrating sphere photometer (Fig. 5.62, line 5).
In lines 1 and 2 corresponding to the spectral curves of the reflection coefficient
of skin at wavelengths of 542, 560, 577 nm, characteristic peaks were observed,
which coincide with the peaks of spectral curve of the blood preparation reflection
(Fig. 5.62, line 5) and the spectrum of blood absorption (Fig. 5.60, line 1).
Artificially caused ischemia leads to the decrease of the amount of oxyhemoglobin
practically to zero. Complete disappearance of characteristic peaks of spectral reflection curve for wavelengths of 542 and 577 nm is registered, and the sign of curvature of spectral reflection curve at a wavelength of 560 nm. Averaged values of
coefficients Q 1 and Q 2 at normal condition equal to 3 and 2, at ischemia—3 and
7, respectively. Thereby, introduced coefficients Q 1 and Q 2 , being independent
Fig. 5.62 Spectral changes of reflection coefficient of skin and human blood. 1, 2—normal in the
first and second testees; 3, 4—ischemia in the first and second testees, respectively. Reprinted from
[87] with permission
5 Laser Specklometer, Speckle-Optical Diagnostics and Laser …
determined by individual reflecting characteristics of skin of every single respondent.
Thus, according to our data, the method of the diagnostics of ischemia suggested by
the authors is not reliable due to a great influence on the results of measurements of
individual characteristics of reflecting features of human skin.
The study of spectral reflection coefficient of skin in vivo on the inner side of a
thumb was conducted at different functional states. The measurements of spectral
curves of reflection coefficient were conducted with the use of the attachment with
integrating sphere photometer at two people. People under test tightly applied to the
aperture for installation of a sample of integrating sphere photometer the area under
study of skin at the inner side of a thumb. Spectral changes of reflection coefficient of
skin at the same conditions (Fig. 5.62, lines 1 and 2 for both testees) were registered.
Artificial ischemia of the skin area was caused by air inflating of air-inflated collar,
which had been put on forearm beforehand, till complete blood flow stops of a limb.
Later, the changes of spectral reflection coefficients of skin were registered, which
are presented in Fig. 5.62 (the line 3 for the first testee and the line 4 for the second
one). Then, a special cuvette with blood preparation was installed into the aperture
for the installation of a sample of integrating sphere photometer (Fig. 5.62, line 5).
In lines 1 and 2 corresponding to the spectral curves of the reflection coefficient
of skin at wavelengths of 542, 560, 577 nm, characteristic peaks were observed,
which coincide with the peaks of spectral curve of the blood preparation reflection
(Fig. 5.62, line 5) and the spectrum of blood absorption (Fig. 5.60, line 1).
Artificially caused ischemia leads to the decrease of the amount of oxyhemoglobin
practically to zero. Complete disappearance of characteristic peaks of spectral reflection curve for wavelengths of 542 and 577 nm is registered, and the sign of curvature of spectral reflection curve at a wavelength of 560 nm. Averaged values of
coefficients Q 1 and Q 2 at normal condition equal to 3 and 2, at ischemia—3 and
7, respectively. Thereby, introduced coefficients Q 1 and Q 2 , being independent
Fig. 5.62 Spectral changes of reflection coefficient of skin and human blood. 1, 2—normal in the
first and second testees; 3, 4—ischemia in the first and second testees, respectively. Reprinted from
[87] with permission