3.4 Holographic Research Methods in Biology and Medicine
273
radiances (Watt ··· cm
−2 sterad
−1 ) of diffusely reflecting objects, onto which the direct
laser radiation gets. The works [254, 255] contain the most detailed information about
MPL, which was obtained in domestic laboratories.
There are two ways of image registration to solve the problem of intracavitary
holographing [243, 244, 256–258]: at the proximal end and the distal one. Based
on theoretical and experimental results, which were conducted on the samples, the
work [258] shows the advantage of holographic endoscopy in comparison to usual
endoscopy, which consists in the possibility of resolution increase.
Hologram copying is a very important issue for medical–biological objects [259–
261]. One should decipher direct copying [261], which is a process close to contact
printing, from getting a secondary hologram when a wave reconstructed by the original hologram interferes with an independently directed reference wave. The obtained
copy is a negative of the initial hologram. But the received image is positive and
identical to the image reconstructed by the original [262–264].
3.4.1 Holographic Recording of Anatomical Preparations
of Vertebrae with Manifestation of Lumbar
Osteochondrosis and Corrosion Preparations of Blood
Vessels of Human Liver
There was shown the possibility of registration of large reflecting holograms of 280
× 406, 400 × 600 mm
2 of some anatomical objects [41, 54, 265]. For these purposes,
there was produced an experimental set-up with the protection from vibrations for
holographing unique medical objects in colliding beams. The necessary accessory
for fixing of different objects was created, which provided their placement both in
horizontal and in vertical planes. 30 mWatt single-mode He–Ne laser LG-38 was
used for hologram recording and photoplates PFG-02 and PFG-03 were used as the
registering medium.
Anatomical objects for hologram recording were human lumbar and jugular vertebrae with such pathologies as osteochondrous calcifications and corrosion preparations of liver blood vessels. The objects were made through common methods of
boring, fixation, cutting in different projections, digestion, preservation, injection of
the vascular system with different self-harding compositions with dyes. To eliminate defects connected with hologram drying, they were placed into baths with 50-,
70-, 80% alcohol solution. Optimal modes were determined, which should be used
for recording and processing holograms and holographic interferograms of human
vertebrae. 3D holographic images give the possibility to examine human vertebrae
in the range of 180° (Fig. 3.46).
Besides, circular holographic recording of human liver corrosion preparation
blood vessels was conducted (Fig. 3.47). As we performed holographic experiments on obtaining highly informative holographic images of living tissue, there
appeared a necessity to form a rather huge amount of beams for illuminating the
273
radiances (Watt ··· cm
−2 sterad
−1 ) of diffusely reflecting objects, onto which the direct
laser radiation gets. The works [254, 255] contain the most detailed information about
MPL, which was obtained in domestic laboratories.
There are two ways of image registration to solve the problem of intracavitary
holographing [243, 244, 256–258]: at the proximal end and the distal one. Based
on theoretical and experimental results, which were conducted on the samples, the
work [258] shows the advantage of holographic endoscopy in comparison to usual
endoscopy, which consists in the possibility of resolution increase.
Hologram copying is a very important issue for medical–biological objects [259–
261]. One should decipher direct copying [261], which is a process close to contact
printing, from getting a secondary hologram when a wave reconstructed by the original hologram interferes with an independently directed reference wave. The obtained
copy is a negative of the initial hologram. But the received image is positive and
identical to the image reconstructed by the original [262–264].
3.4.1 Holographic Recording of Anatomical Preparations
of Vertebrae with Manifestation of Lumbar
Osteochondrosis and Corrosion Preparations of Blood
Vessels of Human Liver
There was shown the possibility of registration of large reflecting holograms of 280
× 406, 400 × 600 mm
2 of some anatomical objects [41, 54, 265]. For these purposes,
there was produced an experimental set-up with the protection from vibrations for
holographing unique medical objects in colliding beams. The necessary accessory
for fixing of different objects was created, which provided their placement both in
horizontal and in vertical planes. 30 mWatt single-mode He–Ne laser LG-38 was
used for hologram recording and photoplates PFG-02 and PFG-03 were used as the
registering medium.
Anatomical objects for hologram recording were human lumbar and jugular vertebrae with such pathologies as osteochondrous calcifications and corrosion preparations of liver blood vessels. The objects were made through common methods of
boring, fixation, cutting in different projections, digestion, preservation, injection of
the vascular system with different self-harding compositions with dyes. To eliminate defects connected with hologram drying, they were placed into baths with 50-,
70-, 80% alcohol solution. Optimal modes were determined, which should be used
for recording and processing holograms and holographic interferograms of human
vertebrae. 3D holographic images give the possibility to examine human vertebrae
in the range of 180° (Fig. 3.46).
Besides, circular holographic recording of human liver corrosion preparation
blood vessels was conducted (Fig. 3.47). As we performed holographic experiments on obtaining highly informative holographic images of living tissue, there
appeared a necessity to form a rather huge amount of beams for illuminating the