140
2 Holographic Microscopy of Phase and Diffuse Objects …
Fig. 2.35 Optical scheme for studying nerve compression when exposed to hydrostatic pressure
by holographic interferometry in reflected light: 1—the laser; 2—the diaphragm; 3–6—the deaf
mirrors; 7, 8—the beam splitters (R = 99% and R = 50%); 9, 10—the light filters; 11—the telescopic
attachment; 12—the lens; 13—the camera with an object; 14—the hologram; 15—the camera.
Reprinted from [136] with permission
ϕ =
2π
λ
L =
2π
λ
{[d(n c − n) + Rn c ] − d(n c − n)}
=
2π
λ
n c R
(2.42)
where d is the shift of camera window; R is the alteration of the nerve radius; n
is the air refraction index; n c is the liquid refraction index where the nerve is placed.
Thus, the nerve compression under high pressure must lead to the “failure” of
interference fringes corresponding to the additional phase incursion ϕ.
The methods described above were tested in three experiments. If the pressure is
in the rate of 0–180 atm. and recording at the accuracy of the optical path difference
is λ/10, such a failure in the nerve interferogram was not found that corresponds to
changes of its radius at the value smaller than 0.02 μm (i.e.,
R
R
≤ 10
−5 ).
This result testifies of the fact, that in processes, which are the basis of nerve function alterations during high pressure application, failures of metabolism processes
play probably the main part, not the mechanical injurious action.
The holographic interferometry method was improved to study a nerve in transmitted light. During hyperbary research of an isolated nerve in transmitted light,
namely, during studying the simultaneous changes in its indices of refraction and
shape, the main problem is still speckle-field decorrelation.
To solve this problem, we developed an interferometry research method of diffused
scattered objects with a slowly changing structure and cylindrical symmetry. The
method is based on optical spatial filtering (OSF) [375] of rays passed through
the object. It is known that applying the OSF-methods at the stage of hologram
recording or during object wave field reconstruction makes it possible to extract
most informative component of their spatial frequency spectra.
2 Holographic Microscopy of Phase and Diffuse Objects …
Fig. 2.35 Optical scheme for studying nerve compression when exposed to hydrostatic pressure
by holographic interferometry in reflected light: 1—the laser; 2—the diaphragm; 3–6—the deaf
mirrors; 7, 8—the beam splitters (R = 99% and R = 50%); 9, 10—the light filters; 11—the telescopic
attachment; 12—the lens; 13—the camera with an object; 14—the hologram; 15—the camera.
Reprinted from [136] with permission
ϕ =
2π
λ
L =
2π
λ
{[d(n c − n) + Rn c ] − d(n c − n)}
=
2π
λ
n c R
(2.42)
where d is the shift of camera window; R is the alteration of the nerve radius; n
is the air refraction index; n c is the liquid refraction index where the nerve is placed.
Thus, the nerve compression under high pressure must lead to the “failure” of
interference fringes corresponding to the additional phase incursion ϕ.
The methods described above were tested in three experiments. If the pressure is
in the rate of 0–180 atm. and recording at the accuracy of the optical path difference
is λ/10, such a failure in the nerve interferogram was not found that corresponds to
changes of its radius at the value smaller than 0.02 μm (i.e.,
R
R
≤ 10
−5 ).
This result testifies of the fact, that in processes, which are the basis of nerve function alterations during high pressure application, failures of metabolism processes
play probably the main part, not the mechanical injurious action.
The holographic interferometry method was improved to study a nerve in transmitted light. During hyperbary research of an isolated nerve in transmitted light,
namely, during studying the simultaneous changes in its indices of refraction and
shape, the main problem is still speckle-field decorrelation.
To solve this problem, we developed an interferometry research method of diffused
scattered objects with a slowly changing structure and cylindrical symmetry. The
method is based on optical spatial filtering (OSF) [375] of rays passed through
the object. It is known that applying the OSF-methods at the stage of hologram
recording or during object wave field reconstruction makes it possible to extract
most informative component of their spatial frequency spectra.