118
2 Holographic Microscopy of Phase and Diffuse Objects …
Fig. 2.20 Study by the method of focusing of light guide properties of nerve fibers grown in
distortion culture of synthetic ganglia. Reprinted from [136] with permission
absorption (less than 6%). If the refraction index has radial dependence n = n(r), it
will work as a cylindrical lens. By measuring intensity distribution P(y) in the plane
behind the fiber (Fig. 2.20c), refraction index profile can be calculated as
n(r ) − n 2 =
n 2
π L
∞
0
t − y(t)
√
t 2 − r 2
dt.
(2.32)
Function y(t) is found as the inverse to the function
t(y) =
y
o
P
y
dy
.
(2.33)
The immersion selection accuracy is 5 × 10
−3 .
The photo of the cell in the physiological solution with the refraction index of
1.334 is shown in Fig. 2.20c, and in Fig. 2.20d—in the medium n 2 = 1.370, where
2 Holographic Microscopy of Phase and Diffuse Objects …
Fig. 2.20 Study by the method of focusing of light guide properties of nerve fibers grown in
distortion culture of synthetic ganglia. Reprinted from [136] with permission
absorption (less than 6%). If the refraction index has radial dependence n = n(r), it
will work as a cylindrical lens. By measuring intensity distribution P(y) in the plane
behind the fiber (Fig. 2.20c), refraction index profile can be calculated as
n(r ) − n 2 =
n 2
π L
∞
0
t − y(t)
√
t 2 − r 2
dt.
(2.32)
Function y(t) is found as the inverse to the function
t(y) =
y
o
P
y
dy
.
(2.33)
The immersion selection accuracy is 5 × 10
−3 .
The photo of the cell in the physiological solution with the refraction index of
1.334 is shown in Fig. 2.20c, and in Fig. 2.20d—in the medium n 2 = 1.370, where