3 Bio-microelectromechanical Systems (BioMEMS) in Bio-sensing …
77
Table 3.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
• PDMS
• Micro-spheres
The PDMS chip was
fabricated through
soft lithography
bound to glass slides
using a plasma
cleaner. The
polystyrene
micro-spheres were
exposed to
streptavidin, and the
resulting conjugates
were bound to a
primary antibody for
initial entrapment of
insulin or insulin
analog
A sample of T1D
patients’ blood was
taken and pumped
through the inlet of
the device. As the
sample flows through
the channel, it is
intersected with
functionalized
micro-spheres and
subsequently
conjugated with
secondary antibody
fluorescently tagged.
The mixture travelled
through outlet channel
to be imaged for the
final readout
Insulin analogs
The device
accurately detected
the insulin levels
within 30s of
injecting sample
into the chip
Cohen (2017)
(continued)
77
Table 3.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
• PDMS
• Micro-spheres
The PDMS chip was
fabricated through
soft lithography
bound to glass slides
using a plasma
cleaner. The
polystyrene
micro-spheres were
exposed to
streptavidin, and the
resulting conjugates
were bound to a
primary antibody for
initial entrapment of
insulin or insulin
analog
A sample of T1D
patients’ blood was
taken and pumped
through the inlet of
the device. As the
sample flows through
the channel, it is
intersected with
functionalized
micro-spheres and
subsequently
conjugated with
secondary antibody
fluorescently tagged.
The mixture travelled
through outlet channel
to be imaged for the
final readout
Insulin analogs
The device
accurately detected
the insulin levels
within 30s of
injecting sample
into the chip
Cohen (2017)
(continued)